【摘要】 目的建立以2,4-二叔丁基苯酚为内标的高效液相色谱法测定丙泊酚血药浓度方法。方法血清样品用乙腈-甲醇（75:25）混合液蛋白沉淀后取上清液进样分析,以2,4-二叔丁基苯酚为内标,采用Ultimate^XB-C₁₈色谱柱（4.6 mm×250 mm,5μm）,以水（H₃PO₄调pH=1.8）-乙腈（70∶30）为流动相,流速1.5 mL/min,检测激发波长270 nm。结果该法专属性高,回收率高,日内日间RSD低。丙泊酚血药浓度在0.2¹0.0 mg/L范围内线性关系良好（r²=0.9998）,最低检测浓度0.2970 mg/L。结论本方法测定丙泊酚浓度方便、快捷、准确度好、精密度高,为开展血药浓度检测、药动学研究提供方法学依据。更多还原

【Abstract】 Objective To establish a detecting method of propofol plasma concentration by HPLC with 2,4-ditert-butyl phenol as internal standard.Methods The supernatant of serum samples were analyzed after protein precipitation by acetonitrile-methyl alcohol（75∶25） mixed liquor.With 2,4-ditert-butyl phenol as internal standard,Ultimate^XB-C₁₈ chromatographic column（4.6 mm×250 mm,5 μm） was used.Water（H₃PO₄,pH=1.8）-acetonitrile（70∶30） was used as mobile phase,flow velocity=1.5 mL/min,and detecting excitation wave length=270 nm.Results This method had high specificity and recovery,with low intra-day and inter-day RSD.When the plasma concentration of propofol was between 0.2¹0.0 mg/L,it showed a good linear relation（r²=0.9998）,and the lower limit of detection was 0.2970 mg/L.Conclusion This method is convenient and speedy,with high accuracy and precision,providing a methodological basis for plasma concentration detection and pharmacokinetic research.更多还原