【摘要】 目的采用高效液相色谱-二极管阵列检测器-蒸发光检测器(HPLC-DAD-ELSD)联用技术,建立苦丁茶冬青叶的指纹图谱。方法岛津C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-水为流动相进行梯度洗脱,体积流量1.0 mL/min,检测波长210 nm,柱温35℃。结果通过10批苦丁茶冬青药材的指纹图谱,建立了共有模式,其中HPLC-DAD指纹图谱共有峰18个,相似度为0.911~0.970,HPLC-ELSD指纹图谱共有峰11个,相似度为0.914~0.962。结论方法精密度、重现性、稳定性好,为客观评价苦丁茶冬青药材的质量提供了科学依据。更多还原

【Abstract】 Objective In this paper,HPLC-DAD-ELSD technique was used to establish the chromatography fingerprint of Ilex kudingcha.Methods Shimadzu C18 column(250 mm × 4.6 mm,5 μm) was used.Acetonitrile-water as the mobile phase; The flow speed was 1.0 m L/min.The detection wavelength was 210 nm and the column temperature was 35 ℃.Results The chromatography fingerprint of Ilex kudingcha from 10 different origins was established.In the chromatography fingerprint with HPLC-DAD of Ilex kudingcha,18 common peaks were damarcated and the similarities of Ilex kudingcha were between 0.911—0.970,and the chromatography fingerprint with HPLC-ELSD of Ilex kudingcha,11 common peaks were damarcated and the similarities of Ilex kudingcha were between 0.914 — 0.962.Chlorogenic acid,kaempferol-3-O-β-D-rhamnoside,isorhamnetin-3-O-β-D-rhamnoside,kudinoside C,kudinoside A,kudinoside E,kudinoside D were confired by LC-MS and binding control.Conclusion The method of precision,reproducibility,stability is accurate,which can be used as the evaluation of the quality of Ilex kudingcha.更多还原