【摘要】 目的研究慢性丙型病毒性肝炎患者外周血单核细胞(PBMC)中2’,5’寡腺苷酸合成酶(2’,5’-Oligoadenylate synthetase,2-5AS)的表达及其传导与转录激活因子(Signal transducer and activator of transcription,Stat)Stat1和Stat2等的水平相关性分析。方法选择60例慢性丙肝患者,抽取外周血分离PBMC。首先测定每份标本的2-5AS活性。然后将所有丙肝患者的PBMC标本按按编号单双数分为对照组和实验组,进行体外培养。其中,对照组PBMC不添加干扰素α(interferon alpha,IFN-α),而实验组PBMC添加1000IU/ml IFN-α。体外培养大约24小时后,测定PBMC中2-5AS活性、Stat1和Stat2等的表达量。结果和对照组相比,实验组PBMC的2-5AS活性明显升高(P<0.05)。实验组PBMC中,在2-5AS活性增高的同时,Stat1和Stat2等表达量也呈现明显增多的趋势(P<0.05)。结论基础的2-5AS活性与加入IFNα刺激培养后Stat1的改变幅度呈负相关(P<0.05);2-5AS基础水平和IFNα刺激后的Stat2表达的变化没有相关性(P>0.05)。更多还原

【Abstract】 Objective To investigate the correlation between 2’, 5’-Oligoadenylate synthetase(2-5AS) activity and Stat1/Stat2 levels in peripheral blood mononuclear cells(PBMC)of patients suffered fromchronic hepatitis C virus(HCV). Methods PBMC from 60 HCV patients were extracted and examined Then PBMC in each sample was optionally divided into two groups, which were the control group and the experimental group. PBMCs in experimental group were cultured in vitro for 24 hours at the presence of 1000 IU/ml of interferon alpha(IFN-α), while PBMCs in control group were cultured without IFN-α. 24 hours later, the activity of 2-5AS and levels of Stat1/Stat2 were assessed. The correlations between basal 2-5AS activity and changes of 2-5AS, Stat1 and Stat2 were evaluated. Results Compared with control group, activity of 2-5AS in experimental group was significantly elevated(P<0.05). And in experimental group, similar to the elevation of 2-5AS activity, Stat1/Stat2 levels were also significantly up-regulated(P<0.05). Conclusions Basal 2-5AS activity was negatively correlated with the changes of Stat1 after IFN-α stimulation(P<0.05), while there was no obvious correlation between basal 2-5AS and Stat2 level after IFN-α stimulation(P>0.05).更多还原