【摘要】 背景:有研究发现,血管紧张素Ⅱ受体拮抗剂对骨的保护作用强于较血管紧张素转换酶抑制剂。目的:观察不同浓度血管紧张素Ⅱ受体拮抗剂厄贝沙坦对小鼠前成骨细胞分化和矿化的影响。方法:选取对数生长期小鼠前体成骨细胞株MC3T3-E1,分4组培养,分别加入含0(对照),0.001,0.01,0.1 mmol/L厄贝沙坦的成骨诱导培养基培养。诱导培养10 d,采用碱性磷酸酶染色观察细胞分化情况;诱导培养21 d,采用茜素红染色观察细胞矿化情况;诱导培养1,4,7,14,21 d,RT-PCR检测细胞成骨细胞内骨钙素、碱性磷酸酶、Runt相关转录因子2mR NA的相对表达量。结果与结论:(1)碱性磷酸酶染色:0.001,0.01,0.1 mmol/L厄贝沙坦组碱性磷酸酶活性均高于对照组(P<0.05),其中以0.01 mmol/L效果最明显;(2)茜素红染色:0.001,0.01,0.1 mmol/L厄贝沙坦组钙结节数量及面积均高于对照组(P<0.05),其中以0.01 mmol/L效果最明显;(3)RT-PCR检测:0.01 mmol/L厄贝沙坦组诱导培养不同时间点的碱性磷酸酶、Runt相关转录因子2、骨钙素mR NA相对表达量均高于对照组(P<0.05);(4)结果表明:0.01 mmol/L厄贝沙坦可显著促进成骨细胞的分化和矿化。更多还原

【Abstract】 BACKGROUND: Angiotensin II receptor antagonists have been found to exerct a stronger protective effect on bone than angiotensin converting enzyme inhibitors. OBJECTIVE: To investigate the effect of different concentrations of irbesartan(angiotensin II receptor antagonist)on the differentiation and mineralization of mouse preosteoblasts. METHODS: Mouse preosteoblast cell lines MC3T3-E1 in logarithmic phase were selected and cultured in the osteogenic induction medium containing 0(control group), 0.001, 0.01, 0.1 mmol/L irbesartan, respectively. Ten days later, the cell differentiation was observed by alkaline phosphatase staining. The mineralization was observed by alizarin red staining after 21 days of culture. m RNA expressions of osteocalcin, alkalinephosphatase and Runt-associated transcription factor 2 in osteoblasts were detected by real-time PCR at 1, 4, 7, 14 and 21 days of culture. RESULTS AND CONCLUSION: The activity of alkaline phosphatase in all the irbesartan groups(0, 0.001, 0.01, 0.1) was higher than that in the control group(P < 0.05), which was the most obvious in 0.01 mmol/L. The number and area of calcium nodules in each irbesartan group were significantly higher than those in the control group(P < 0.05), especially in 0.01 mmol/L. Compared with the control group, 0.01 mmol/L irbesartan significantly upregulated the m RNA expressions of osteocalcin, alkaline phosphatase and Runt-associated transcription factor 2(P < 0.05). These results suggest that 0.01 mmol/L irbesartan significantly promotes the differentiation and mineralization of osteoblasts.更多还原