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Targeting BCRP/ABCG2 by RNA Interference Enhances the Chemotherapy Sensitivity of Human Colon Cancer Side Population Cells

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【作者】 胡均； 李健； 岳欣； 王家仓； 王俊锋； 柳建中； 孔大陆；

【Author】 Jun HU;Jian LI;Xin YUE;Jia-cang WANG;Jun-feng WANG;Jian-zhong LIU;Da-lu KONG;Department of Colorectal Cancer Surgery,National Clinical Research Center for Cancer,Key Laboratory of Cancer Prevention and Therapy of Tianjin,Tianjin’s Clinical Research Center for Cancer,Tianjin Medical University Cancer Institute and Hospital;Department of Lymphoma,State Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Disease Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College;

【机构】 Department of Colorectal Cancer Surgery,National Clinical Research Center for Cancer,Key Laboratory of Cancer Prevention and Therapy of Tianjin,Tianjin’s Clinical Research Center for Cancer,Tianjin Medical University Cancer Institute and Hospital； Department of Lymphoma,State Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Disease Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College；

【摘要】 Relapse and metastasis are frequent in colon cancer and may be linked to stem cell characteristics.This study isolated side population(SP) cells from a colon cancer cell line(Colo-320) and examined their self-renewal and differentiation abilities.Compared to non-SP(NSP) cells,SP colon cancer cells were more tumorigenic in vivo and exhibited more invasive characteristics and a greater ability to form colonies.Additionally,more cells were in G0/G1 phase and more highly expressed the multidrug resistance protein BCRP/ABCG2.We achieved enhanced chemotherapy sensitivity by transfecting SP cells with a hairpin-like,small interfering RNA(si RNA) eukaryotic expression plasmid targeting BCRP/ABCG2.

【Abstract】 Relapse and metastasis are frequent in colon cancer and may be linked to stem cell characteristics.This study isolated side population(SP) cells from a colon cancer cell line(Colo-320) and examined their self-renewal and differentiation abilities.Compared to non-SP(NSP) cells,SP colon cancer cells were more tumorigenic in vivo and exhibited more invasive characteristics and a greater ability to form colonies.Additionally,more cells were in G0/G1 phase and more highly expressed the multidrug resistance protein BCRP/ABCG2.We achieved enhanced chemotherapy sensitivity by transfecting SP cells with a hairpin-like,small interfering RNA(si RNA) eukaryotic expression plasmid targeting BCRP/ABCG2.

【关键词】 side population cells； colon cancer； chemotherapy sensitivity； RNAi； bcrp/abcg2；
【Key words】 side population cells； colon cancer； chemotherapy sensitivity； RNAi； bcrp/abcg2；
【基金】 supported by grants from the National Nature Science Foundation of China(No.81101870);the National Key Clinical Specialist Construction Programs of China(No.2013-544);the Key Programs of National Health and Family Planning Commission of Tianjin(No.16KG127)
  • 【文献出处】 Journal of Huazhong University of Science and Technology(Medical Sciences) ,华中科技大学学报(医学英德文版) , 编辑部邮箱 ,2017年02期
  • 【分类号】R735.35
  • 【被引频次】9
  • 【下载频次】31
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