【摘要】 目的:探索乙型肝炎病毒核心蛋白区(C区)变异在隐匿性乙型肝炎病毒感染(OBI)中的作用。方法:从2例献血者OBI的样品中扩增得到C区基因构建乙型肝炎病毒C区突变型质粒,检测各突变型质粒在Hu H7细胞中HBs Ag和HBe Ag的水平。Balb/c小鼠高压尾静脉注射突变型质粒检测其血清HBs Ag和HBe Ag的水平,免疫组织化学检测肝组织HBc Ag的表达及分布情况,RT-PCR检测肝组织HBV-RNA的水平。结果:与野生型相比,C区突变型显著性下调HBe Ag和HBc Ag的表达水平,对HBs Ag表达水平和HBV-RNA的水平无显著性作用。结论:OBI患者体内的乙肝病毒C区变异改变HBV病毒的HBe Ag和HBc Ag的表达水平,对OBI的形成具有重要作用。更多还原

【Abstract】 Objective To investigate the role of mutations in C region that may contribute to occult hepatitisB virus infection.Methods C genes were amplified from two OBI blood donor samples respectively.Plasmids withmutations in C region of hepatitis B virus were constructed by overlapping PCR.HBs Ag and HBe Ag in Huh7 cells andin the serum of Balb/c mice were detected by CMLA.HBc Ag in liver tissue was detected by immunohistochemistry,while HBV-RNA was tested by RT-PCR.Results Mutations in C region significantly reduced the expression level ofHBe Ag and HBc Ag,but had no significant effect on HBs Ag and HBV-RNA.Conclusion The mutations in C regionaffect the expression level of HBe Ag and HBc Ag,which may play an important role in the occurrence of OBI.更多还原