【摘要】 鲍鱼希瓦氏菌有望用于对虾养殖和海水石油污染的治理。为探索鲍鱼希瓦氏菌的最优培养基和培养条件,运用单因素实验和正交实验对发酵条件进行优化,并在70 L发酵罐上进行扩大培养。最优培养基为蔗糖6%,蛋白胨2.5%,碳酸钙0.7%,初始p H 6.8。最优摇瓶培养条件为温度30℃,转速130 r/min,装液量100 m L/500 m L,接种量6%,收获时间24 h。70 L发酵罐生物量达到1.14×1011cfu/m L。鲍鱼希瓦氏菌发酵条件的优化为其工业生产提供了实验数据支持。更多还原

【Abstract】 Shewanella haliotis has potential biotechnological applications in shrimp culturing and oil pollution abatement. To determine the optimal fermentation medium and culture conditions of Shewanella haliotis,single factor experiments and orthogonal design were conducted. Further confirmation was carried out in 70 L bioreactor under the optimal medium and culture conditions. The results showed that the optimal medium was6% sucrose,2. 5% peptone and 0. 7% CaCO₃ at initial p H 6. 8,and optimal culture conditions in shaking flask were 30 ℃,130 r / min,100 m L /500 m L loading volume,6% inoculum size and 24 h fermentation cycle. The average of final biomass in 70 L bioreactor reached 1. 14 × 1011 cfu / m L. The fermentation optimization provides experiment data for further industrial production.更多还原