【摘要】 以分离自东北自然发酵大酱中的一株耐盐植物乳杆菌FS5-5(Lactobacillus plantarum FS5-5)为实验对象,在转录水平上对该菌株盐胁迫相关基因表达进行了研究。结果表明:在对数生长期,表达显著下调的共29个基因,分别为参与碳水化合物转运和代谢的4个基因,氨基酸转运和代谢的9个基因,维生素代谢的3个基因,核苷酸代谢的6个基因,遗传信息翻译、核糖体结构和形成的7个基因;表达显著上调的有参与碳水化合物转运和代谢的4个基因。这些变化可能与L.plantarum FS5-5的盐胁迫机制密切相关。选取参与维生素代谢的3个基因和核苷酸代谢的1个基因,通过实时定量聚合酶链式反应对转录组学结果进行验证,结果表明两种方法中基因表达趋势一致。实验对L.plantarum FS5-5的盐胁迫反应进行了比较全面的分析,为提高工业生产中菌株的耐受性提供了理论依据。更多还原

【Abstract】 This investigation studied the expression of salt stress-related genes in Lactobacillus plantarum FS5-5, a salt-tolerance strain isolated from naturally fermented miso in northeastern China, at the level of transcription. Results showed that in the logarithmic growth phase, 29 genes were significantly down-regulated, including four ones involved in carbohydrate transport and metabolism, nine ones involved in amino acids transport and metabolism, three ones involved in vitamin metabolism, six ones involved in nucleotide metabolism and seven ones involved in genetic information translation, ribosomal structure and biogenesis. Four genes involved in carbohydrate transport and metabolism were significantly upregulated, which might be closely associated with salt stress resistance of L. plantarum FS5-5. Three genes involved in vitamin metabolism and one gene involved in nucleotide metabolism were selected to be analyzed by real-time fluorescent quantitative polymerase chain reaction and the results showed that the expression of these genes exhibited the same trend in these two methods. The comprehensive analysis of the mechanism of action of L. plantarum FS5-5 to resist salt stress presented in this study which would provide a theoretical basis to improve the tolerance of strains in industrial production.更多还原