【摘要】 目的利用微切助互作技术优化龙牙百合鳞茎提取总黄酮的工艺方法。方法采用微晶纤维素为助剂,龙牙百合鳞茎为主要原料,总黄酮提取率为指标,通过单因素和正交试验优化龙牙百合鳞茎总黄酮的最优提取参数。结果总黄酮提取工艺条件为:微晶纤维素添加量1.5%(m:m),提取时间35min,提取温度50℃,乙醇体积分数60%(V:V)。该条件下总黄酮提取率为5.98%,是热回流法的2.26倍,且提取总时长由122 min缩短到62min,以60%乙醇作提取溶剂,大大减少了有机溶剂使用。结论微切助互作技术是提取龙牙百合鳞茎总黄酮的一种高效、低成本方法,具有很好的应用前景。更多还原

【Abstract】 Objective To optimize production process of total flavonoids extraction from Longya lilium bulb by press-shear assisted interaction(PAI) technology. Methods The optimum parameters of total flavonoids were investigated using microcrystalline cellulose as additive, Longya lilium bulb as materials, and total flavonoids yield as an index in single factor test and orthogonal test. Results The extraction conditions of total flavonoids were as follows: microcrystalline cellulose dosage was 1.5%(m:m), extraction time was 35min, extraction temperature was 50℃ and ethanol volume fraction was 60%(V:V). Under the optimal conditions, the total flavonoids of Longya lilium bulb was up to 5.98%, which was 2.26 times as that of hot reflux, the total time was shortened from 122min to 62min, and the use of organic solvents was greatly reduced. Conclusion PAI technology exerts an efficient and low-cost method for extraction of total flavonoids from Longya lilium bulb, which has a good application prospect.更多还原