【摘要】 目的探讨华支睾吸虫(Cs)硫氧还蛋白过氧化物酶(TPx)对脂多糖(LPS)致脓毒症小鼠的保护作用。方法 80只Balb/c雄性小鼠随机分成两组,腹腔注射LPS(4 mg/kg)制备脓毒症小鼠模型,造模后30 min尾静脉分别注射PBS、rCsTPx(2.5 mg/kg),观察注射后各组小鼠72 h死亡率;重复实验,每组分别于实验后的3、6、9、12、24、48、72 h"安乐死"处死小鼠,收集血清并取24 h组织用于病理切片观察。结果尾静脉注射CsTPx治疗的小鼠生存率由10%提高到40%;检测血清谷草转氨酶(AST)和谷丙转氨酶(ALT)水平在造模后6、9、12、24 h明显下降,并且血清中炎症因子IL-6在3、6、9 h也明显下调,差异均有统计学意义;组织HE染色揭示CsTPx治疗组小鼠肾组织损伤减轻,炎症细胞浸润也减少。结论 rCsTPx对LPS致脓毒症小鼠有较好的保护作用。更多还原

【Abstract】 Objective To study the protective effects of recombinant Clonorchis sinensis TPx on LPS-induced sepsis mice.Methods The total of 80 male Balb/c mice were injected intraperitoneally(i.p.)with 4 mg/kg of LPS and randomly divided into two groups. The control group was injected with phosphate buffer saline and the treatment group was injected thioredoxin peroxidase(2.5 mg/kg)via the caudal vein 30 min after LPS injection. Their mortality of 72 hour were observed. The specimen was collected after the mice were killed at 3,6,9,12,24,48 and 72 h. The AST and ALT levels,cytokine and histological evaluation were investigated. Results Thioredoxin peroxidase(TPx)of Clonorchis sinensis increased the survival rates of mice from 10% to 40%. In addition,there were significant decreased on AST and ALT levels at 6,9,12 and 24 h;down-regulation of IL-6 levels at 3,6 and 9 h were observed in these mice. Tissues HE staining revealed that Cs TPx treatment reduced renal injury and inflammatory cell infiltration. Conclusion The recombinant thioredoxin peroxidase(TPx)could have a favorable effect on LPS-induced sepsis mice.更多还原