【摘要】 目的调查韶关市分散式供水中大肠埃希菌的污染及其耐药情况,及时发现水性传染源,为保障公共卫生安全提供科学依据。方法采集市内山泉水、井水、储水池水、水库水和溪(河)水等5种水样共计120份,依据GB/T5750-2006《生活饮用水标准检验方法》进行菌落总数、总大肠菌群、耐热大肠菌群、大肠埃希菌4个微生物项目的检测,对检出的大肠埃希菌进行抗生素耐药性检测,并对实验结果进行统计分析。结果 120份水样中有42份微生物学指标均合格,总合格率为35.0%,检出大肠埃希菌42株,其中7株为致腹泻大肠埃希菌。85.7%的大肠埃希菌对21种抗生素的一种或几种具有耐药性,42.9%的菌株呈多重耐药性,5株为产ESBLs大肠埃希菌。结论韶关市分散式供水的水质情况不容乐观,不同水源中存在不同比例的致病性和耐药性大肠埃希菌,市民应谨慎选择饮用水源,饮用前应消毒处理。相关部门应加强管理和提供健康教育,确保居民饮水安全。更多还原

【Abstract】 Objective To investigate the contamination and antibiotic resistance of Escherichia coli in decentralized water supply in Shaoguan.To discover water-borne sources of infection in time,in the purpose of providing scientific basis for the protection of public health security. Methods To collect 5 kinds of 120 water samples, including spring water, well water,storage water,reservoir water and streams. All samples were tested on 4 microbiological items according to GB/T 5750-2006 Standard Test Method For Drinking Water, including the total number of colonies, total coliform, thermotolerant coliform bacteria and Escherichia coli. And the antibiotic resistance of Escherichia coli was detected. And then the experimental results were analyzed statistically. Results Totally 42 samples were qualified of microbiological indexes in the 120 samples,the total qualified rate was 35.0%.42 strains of Escherichia coli were detected. Among them, 7 strains of diarrheagenic Escherichia coli were identified. 85.7% of Escherichia coli were resistant to one or more of the 21 antibiotics, and 42.9% of the strains showed multiple resistance, and 5 strains were ESBLs-producing Escherichia coli. Conclusion The quality of decentralized water supply in Shaoguan is not optimistic.The proportions of pathogenicity and antibiotic resistant Escherichia coli in different water sources were different.The public should be careful to choose drinking water, and to take disinfection measures before drinking the water. Relevant departments should strengthen the managements and provide health education to ensure the safety of drinking water.更多还原