【摘要】 为了研究鼠伤寒沙门氏菌对肠道屏障的破坏作用,应用小鼠结肠病理组织切片技术、蓝色葡聚糖2000渗透量检测并结合transwell细胞层模型和荧光定量PCR方法探寻鼠伤寒沙门氏菌破坏肠道屏障的可能机制。病理切片结果显示:鼠伤寒沙门氏菌感染可导致肠上皮细胞受损,Babl/c小鼠小肠外蓝色葡聚糖2000渗透量在2、4和6h升高(P<0.05),昆明小鼠小肠外其渗透量在2和4h时显著升高(P<0.005);细胞跨膜电阻值降低(P<0.05);细胞间紧密连接蛋白ZO-1、Claudin-1基因mRNA转录水平下调(P<0.005)。结果表明,鼠伤寒沙门氏菌能够通过下调肠上皮细胞紧密连接蛋白的表达来破坏细胞间的紧密连接结构,从而达到破坏肠道屏障的作用。补充阐明了鼠伤寒沙门氏菌感染对肠道屏障的影响,对揭示研究鼠伤寒沙门氏菌的致病机制提供一定依据。更多还原

【Abstract】 In order to investigate the break of intestinal barrier after treated with Salmonella typhimurium,the intestinal histopathologic sections and dextran blue 2000 concentration,together with the transwell tight junction structure model and RT-qPCR technology,were used to seek the mechanism.The results of histologic section were showed that the intestinal epithelial cell and intestinal barrier were broken after treated with Salmonella typhimurium,and the relative concentration of dextran blue 2000 was increased in Babl/c(P<0.05)and Kunming mice(P<0.005)respectively after treated in 2hand 4h.The relative TEER value was decreased in Caco-2 model(P<0.05)and the mRNA expressing(ZO-1,Claudin-1)was down-regulated(P<0.001).All those above demonstrate that Salmonella typhimurium can make the genes of tight junction protein related genes down-regulate expression,and then intestinal epithelial cell tight junction structure and intestinal barrier were broken.This research demonstrated the effect of intestinal tract treated by Salmonella typhimurium,and it is a significant for the deep study on pathogenicity of Salmonella typhimurium.更多还原