【摘要】 目的明确牡丹江地区女性宫颈感染人乳头瘤病毒(Human Papillomavirus,HPV)基因型的分布特征。方法应用PCR-反向点杂交法(PCR-RDH)对牡丹江地区642例女性宫颈上皮细胞标本进行23种HPV基因型的分型检测。结果642例中共检出21种HPV基因型(HPV73、82型未检出),HPV感染阳性者226例,总感染率为35.20%;高危型HPV感染126例,阳性率为19.63%,排列前四位的高危基因型依次为HPV16、52、58、51型,低危型感染23例,阳性率为3.58%,以HPV6、43型为主,混合型感染77例,阳性率为11.99%;感染类型以单一HPV基因型感染为主,阳性率为23.20%,占65.93%,其中二重HPV感染阳性率为6.86%,占19.47%,三重及以上混合HPV感染阳性率为5.14%,占14.60%;≤20岁、21~30岁、31~40岁、41~50岁、51~60岁和>60岁6个年龄组中HPV感染阳性率分别为28.57%、40.91%、39.62%、32.14%、32.26%和23.81%。结论 HPV16、52、58、51型是牡丹江地区女性宫颈感染HPV的主要高危基因型,HPV感染率以21~30、31~40岁组中最高,分别为40.91%、39.62%。更多还原

【Abstract】 Objective To clear the distribution characteristics of human papillomavirus(Human Papillomavirus,HPV) genotype.Methods The HPV were detected and typed by PCR-reverse dot blot hybridization(PCR-RDH) in 642 cervical cell samples in Mudanjiang district.Results A total of 642 cases of female cervical epithelial cells detected 21 HPV genotypes(HPV73,82 not detected).HPV infection was 226 cases and the total HPV infection rate was 35.20%; High-risk type HPV infection was 126 cases and the positive rate was 19.63%; the arrangement of the top four high-risk genotypes was HPV16,52,58,51.Low-risk type infection was 23 cases and the positive rate was 3.58%,giving priority to with HPV6,43.A mixed type infection was 77 cases and the positive rate is 11.99%; Infection type is given priority to a single HPV genotypes infection and the positive rate was 23.20%,accounted for 65.93%,in which a double type infection positive rate was 6.86%,accounted for 19.47%; more than triple and multiple type infection positive rate was 5.14%,accounted for 14.60%; the positive rate of HPV were 28.57%,40.91%,39.62%,40.91%,32.26% and 23.81% long with the age group of≤20、21 ~ 30、31 ~ 40、41 ~ 50、51 ~ 60 and > 60 years,respectively.Conclusion HPV16,52,58,51 are the main high-risk genotypes of 642 women in Mudanjiang district.HPV infection rate was the highest in 21~ 30,31 ~ 40 years old group,40.91%,39.62%,respectively.更多还原