【摘要】 目的:确定白芍总苷(Total Glucosides of Paeony,TGP)对小鼠特应性皮炎的保护作用。方法:利用2,4-二硝基氯苯(DNCB)建立Nc/Nga小鼠实验性特应性皮炎模型。30只小鼠分为3组:正常对照组,模型组,实验组(TGP治疗),每组10只。实验组连续灌胃用药2周,分别于第8 d、15 d测量各组小鼠背部皮损厚度和皮肤质量,并利用ELISA法分别检测第8 d、15 d血清中细胞因子IFN-γ,IL-4,IL-5和IgE的表达。结果:白芍总苷治疗第8 d、15 d,皮损厚度变薄及皮损皮肤质量明显减小(P<0.05),血清细胞因子IL-4、IL-5和IgE水平明显低于模型组,IFN-γ水平明显高于模型组(P<0.05)。结论:白芍总苷可能通过促进IFN-γ分泌,下调炎症因子IL-4、IL-5水平以及抑制IgE表达从而对小鼠特应性皮炎产生保护作用。更多还原

【Abstract】 Objective: To determine the effect of total glucosides of paeony( TGP) on atopic dermatitis in mice. Methods: An atopic dermatitis model was established on the back of NC / Nga mice with DNCB. Thirty mice were divided into 3 groups: control group,model group and TGP group( 10 mice in each group). The mice of TGP group were treated by TGP for 14 days. The levels of serum cytokines including IL-4 and IL-5,IFN-γ and IgE were detected with ELISA kit at day 8th and 15 th. Results: TGP could significantly decrease the skin thickness and weightness at 8 days and 15 days( P<0.05). The levels of IL- 4,IL- 5 and IgE were significantly decreased,while the level of IFN-γ was increased in TGP group,compared with other groups.Conclusion: TGP has protective effects on atopic dermatitis in mice,which may be through inhibiting production of IgE,IL-4 and IL-5,and promoting the secretion of IFN-γ.更多还原