【摘要】 目的以国内广布种日本三角涡虫(Dugesia japonica)为受试动物,探讨三类生物因子对涡虫生殖和再生的影响。方法选择与生殖相关的激素(雌二醇、甲基睾丸素),生物胺(5-羟色胺、多巴胺)和D-氨基酸(D-色氨酸、D-精氨酸、D-苯丙氨酸)对日本三角涡虫进行急性毒性实验,用SPSS 17.0统计学软件计算出适宜的实验浓度,对整体及再生涡虫进行培养,观察并记录实验现象。结果雌二醇、5-羟色胺、D-色氨酸和D-苯丙氨酸处理组分别引起20%、40%、20%和20%涡虫卵巢及交配器官的发育;而多巴胺则引起40%~50%涡虫生殖腺退化。另外,5-羟色胺处理组涡虫头部再生速度加快,而多巴胺处理组则头部再生速度减慢。结论雌二醇、5-羟色胺、D-色氨酸和D-苯丙氨酸能够诱导涡虫生殖腺的发育,并且5-羟色胺促进涡虫头部再生;多巴胺则引起涡虫生殖腺退化并对涡虫再生有抑制作用。更多还原

【Abstract】 Objective To investigate the effects of the three reproductive-relative factors on gonads and regeneration in planarians.Methods In this study,the planarians Dugesia japonica were cultured with three reproductiverelative factors:the hormones [estradiol(E2),methyltestosteronum(MT) ],biogenic amine [5-hydroxy-tryptamine(5-HT),dopamine(DA) ]and D-amino acids(D-tryptophan,D-arginine,D-phenylalanine).The cultured concentration was calculated by SPSS17.0 after the acute toxicity experiment,and the experimental phenomena were recorded.Results E2,5-HT,D-tryptophan and D-phenylalanine improved the gonads development,which proportions were 20%,40%,20%,and 20% respectively,but DA made the gonads atrophy in 40% or 50% planarians.Besides,5-HT increased speed of regeneration,while DA inhibited it during planarians regeneration.Conclusion E2,5-HT,D-tryptophan and Dphenylalanine improved the gonads development,and 5-HT increased speed of regeneration;DA inhibited the gonads and regeneration.更多还原