【摘要】 目的探讨线粒体转录终止因子(MTERFs)蛋白家族在组织和细胞中的表达以及在神经毒素MPTP/MPP+诱导下的表达变化。方法取正常C57BL小黑鼠的脑、肝、肾组织,以及人神经母细胞瘤细胞SH-SY5Y、人心肌细胞HCM、人肝细胞L-02,检测MTERFs的蛋白表达情况;通过MPTP/MPP+构建帕金森病(PD)动物/细胞模型,检测MTERFs的蛋白表达变化情况。结果 MTERFs在小鼠脑中和SH-SY5Y细胞中蛋白表达水平较高;MPTP/MPP+诱导的PD模型中,MTERF3蛋白表达水平明显降低。结论在小鼠脑和SH-SY5Y细胞中,MTERFs表达较高;MPTP/MPP+诱导下MTERF3蛋白表达明显降低,影响了线粒体转录水平,这可能参与了帕金森病发生的过程。更多还原

【Abstract】 Objective To investigate the expression of mitochondrial transcription termination factors(MTERFs)in various tissues and cells,and the changes of MTERFs induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)/1-methyl-4-pheny lpyridine(MPP+).Methods Western blotting was used to measure the expression of the MTERFs in brain,liver,kidney of C57 BL mice and in human SH-SY5 Y,HCM,L-02 cells.MPTP/MPP+was injected to imitate PD animal/cell models.The changes of MTERFs protein were detected through western blotting.Results The expression of MTERFs was higher in mice brain and SH-SY5 Y cells.In PD mouse model induced by MPTP,MTERF3 reduced significantly.Conclusion The expression of MTERFs was higher in mouse brain and SH-SY5 Y cells.After induced by MPTP/MPP+,MTERF3 decreased obviously,which increased transcription level and might involve in a process of Parkinson’s disease.更多还原