【摘要】 本文利用一种具有H₂O₂催化活性的Cu-MOF[Cu₃（BTC）₂（H₂O）₃,简称HKUST-1],构建了以邻苯二胺（OPD）为颜色指示分子的比色传感体系,实现了对H₂O₂和多巴胺（DA）的快速灵敏检测。HKUST-1起到催化H₂O₂氧化OPD的作用,反应体系能够呈现出显著的颜色变化。在优化条件下,415nm处的吸收峰强度与H₂O₂浓度呈双线性关系,线性范围分别为10⁵0 mmol/L和50¹00 mmol/L,相对标准偏差分别为0.9947和0.9995,最低检出限为1.29mmol/L。由于DA能抑制H₂O₂氧化OPD,因此比色传感体系还可以用于快速检测DA,线性范围分别为0.25⁵μmol/L和2.5²5μmol/L,相对标准偏差分别为0.9783和0.9705,最低检出限为0.262μmol/L。该项工作拓展了Cu-MOFs材料在生物分子催化和生物传感方面的应用。更多还原

【Abstract】 In this work,a colorimetric biosensing platform for H₂O₂ and dopamine（DA） was constructed based on the catalytic activity of one type of Cu-MOF [Cu₃（BTC）₂（H₂O）₃,HKUST-1]. The color of o-phenylenediamine（OPD） can be changed significantly because of the oxidation of H₂O₂ catalyzed by HKUST-1. As a result,H₂O₂ and DA were detected rapidly and sensitively by our system. Under the optimum conditions,two linear relationships between the intensity of absorption peak at 415 nm and the concentration of H₂O₂（from 10 to 50 mmol/L and from 50 to 100 mmol/L,R = 0. 9947 and 0. 9995） were obtained with the detection limit of 1. 29 mmol/L. Besides,DA can resist the oxidation of OPD from H₂O₂,this platform was further used for rapid detection of DA with two linear ranges（from 0. 25 to 5μmol/L and from 2. 5 to 25μmol/L,R = 0. 9783 and 0. 9705）,and the detection limit was 0. 262 μmol/L.Therefore,this work may broaden the applications of Cu-MOFs in biological molecular catalysis and biosensing fields.更多还原