【摘要】 目的检测在膀胱癌中SOX9的表达情况,探讨其在膀胱癌中发生、发展可能起的作用。方法选取近5年在广州市花都区人民医院泌尿外科住院行膀胱癌手术的80例患者的病理标本,以60例膀胱癌组织作为研究组,20例膀胱癌旁组织作为对照组,采用免疫组织化学染色及Western blot方法测定各组研究对象中SOX9蛋白的表达水平。结果 SOX9在膀胱癌组织中阳性表达区域主要出现在细胞核,在膀胱癌旁组织中阳性表达区域主要在细胞质和细胞核。SOX9在膀胱癌中的表达比在膀胱癌旁中的高,SOX9在膀胱癌中是癌与非癌的表达为差异有统计学意义(P<0.001),而与年龄、性别和临床病理分期无关(P>0.05)。结论 SOX9在膀胱癌中表达升高,且在癌与非癌中表达有差异,可能成为膀胱癌新的诊断标志物。更多还原

【Abstract】 Objective To detect the expression of SOX9 in urinary bladder carcinoma and explore its possible role in the occurrence and development of urinary bladder carcinoma.Methods Pathological samples of 80 patients who received surgery due to urinary bladder carcinoma in the Urinary Surgery Department of Huadou People’s Hospital were collected for the study.Carcinoma tissues from 60 cases were used as the research group,and benign tissues from another 20 cases were used as the control group.The expressions level of SOX9 were detected in the research subjects of the 2 groups by using immunohistochemistry(IHC) and Western blot(WB).Results Positive immunostainings of SOX9 were observed in the nucleus of carcinoma cells,while the SOX9 positive immunostainings in benign tissues could be noticed in the cytoplasm and nucleus of the surrounding gland epithelial cells.The expression level of SOX9 in bladder carcinoma was higher as compared with that of the benign tissue surrounding carcinoma,and the expression level of SOX9 in bladder carcinoma was higher than that of the benign tissues surrounding carcinoma.Statistical significance could be seen in the expression level of SOX9,when comparisons were made between carcinoma tissue and non-carcinoma tissue(P<0.001).However,clinical pathological grading seemed to have no association with age and gender(P>0.05).Conclusion The expression level of SOX9 elevated in bladder carcinoma,and significant differences could be seen in the expressed levels of carcinoma and benign tissues,which might be a potential biomarker in the diagnosis of bladder carcinoma.更多还原