【摘要】 目的 UPLC法测定注射用左卡尼汀含量,缩短检验周期,提高检验效率。方法利用超高效液相色谱法,BEH C18色谱柱（50×4.6mm,1.7μm,waters公司）,乙腈-磷酸氢二铵缓冲溶液（16∶84）为流动相,柱温为35℃;流速0.3m L·min^-1,检测波长为225nm。结果左卡尼汀在质量浓度0.8¹.2mg·m L^-1范围内线性关系良好（r=0.9995）,平均回收率为99.8%。结论本方法简单、快速、准确,可用于注射用左卡尼汀的含量测定。更多还原

【Abstract】 OBJECTIVE To reduce content examination cycle and improve examination efficiency of the content determination of levocarnitine solution for injection by UPLC. METHODS UPLC method employed an BEH C18 chromatographic column（ 50 × 4. 6nm,1. 7μm,waters） was used; acetonitrile-diammonium hydrogen phosphate buffer solution（ 16∶ 84） was used as mobile phase,temperature of column was 35℃; flow rate was 0. 3m L·min^-1,detection wavelength was 225 nm. RESULTS Levocarnitineoral solution had a good linear relationship in the range of0. 8 1. 2mg·m L^-1（ r = 0. 9995）; the average recovery rate was 99. 8%. CONCLUSION The method is easy,quick and accurate and can be used in content examination of levocarnitineoral solution for Injection.更多还原