【摘要】 目的通过对重庆市2011-2016年分离的风疹病毒进行基因型别鉴定及系统进化分析,为预防和控制风疹提供分子流行病学依据。方法收集重庆市2011-2016年风疹病毒核酸检测阳性病原学标本进行病毒分离,通过序列测定鉴定其基因型别;与GenBank中风疹各型别参考毒株及其他省市所获毒株进行进化树构建和遗传距离分析。结果 2011-2013年风疹毒株主要为1E基因型(94/103);2014-2016年主要为2B基因型(50/56),仅2014年有1株2A基因型毒株。重庆株系与其他省市毒株核苷酸同源性1E型为97.63%~99.73%,平均98.90%;2B型为95.77%~99.73%,平均98.30%。系统树上重庆毒株并未与其他省市株系相互隔离。结论重庆市风疹优势基因型近年已经逐渐由1E型替代为2B型。重庆市的风疹病毒基因型在不断变化,优势株与其他省市呈共同进化状态。更多还原

【Abstract】 Objective To identify and analyze the genetic characterization of rubella viruses isolated in Chongqing from 2011 to 2016,so as to provide molecular epidemiological evidence for rubella prevention and control.Methods Virus samples isolated from Chongqing city from 2011 to 2016 for detection of rubella virus nucleic acid were collected,and their genotypes were identified by sequence analysis.Phylogenetic tree construction and genetic distance analysis were carried out among rubella virus types,reference strains and other provinces and cities in GenBank.Results The predominant genotype from 2011 to 2013 was 1E(94/103),from2014 to 2016 was 2B(50/56).Only one strain of genotype 2A was found in 2014.The homology of 1E genotype between Chongqing and other provinces was 97.63% to 99.73%,with an average of 98.90%.The homology of 2B genotype between Chongqing and other provinces was 95.77% to 99.73%,with an average of 98.30%.The Chongqing rubella viruses strains didn′t isolate form other province strains of China in phylogenetic tree.Conclusion The predominant genotype 1E of rubella virus in Chongqing was gradually replaced by 2B in recent years.The rubella virus genotypes in Chongqing are changing,and the dominant strains are in a state of coevolution with other provinces and cities.更多还原