【摘要】 为提高非洲菊增殖培养的效率并降低生产能耗,通过发光二极管(LED)设置不同比例的红蓝光组合,研究光质对非洲菊离体培养增殖阶段的影响,通过对丛生苗形态指标和生理生化指标的测定,筛选出最适LED光质条件,并初步探讨其生理机制。结果显示:(1)不同LED光质条件下,非洲菊组培苗的增殖系数差异显著,其中RB4(红蓝光比例为4∶1)处理显著高于其他处理。(2)丛生苗的株高在复合光处理下显著低于R(单一红光)和B(单一蓝光)处理;鲜重和干重在红蓝复合光RB2、RB4、RB8(红蓝光比例分别为2∶1、4∶1和8∶1)处理下均显著高于R、B和对照处理。(3)RB4处理下丛生苗的可溶性糖含量显著高于其他处理,比对照提高了89.62%。(4)LED复合光处理下丛生苗的丙二醛含量显著低于对照,并呈现随红光比例增加而不断降低的趋势;RB2、RB4和RB8处理的超氧化物歧化酶活性显著低于其他处理;除R处理外,其他LED光质处理的丛生苗过氧化氢酶活性均显著高于对照,并以RB4处理丛生苗过氧化氢酶活性最强。研究表明,RB2、RB4和RB8处理有助于提高非洲菊组培苗的增殖系数和干鲜重,并能够促进可溶性糖含量的增加,增强植株自身对活性氧的解毒能力,减轻植株的膜脂过氧化作用,且以RB4处理表现最优,其非洲菊丛生苗的增殖系数和可溶性糖含量显著高于其他处理,干鲜重和过氧化氢酶活性均为最高,丙二醛含量显著低于除单一红光外的其他处理。更多还原

【Abstract】 In order to improve the efficiency and reduce the energy consumption,we set up different proportions of red and blue light by light emitting diodes(LEDs).The effects of light qualities on proliferation culture of Gerbera jamesonii Bolus plantlets were investigated based on the morphological and physiological and biochemical indexes.The most suitable LED light condition was selected,and the physiological mechanism was discussed preliminarily.The results showed that:(1)the multiplication rate of gerbera plantlets had significant difference under different treatments of LED light qualities.It was the highest multiplication rate under RB4(the ratio of red to blue light was 4∶1)during treatments.(2)The plantlet height under composite light significantly lower than that of R(red light only)and B(blue light only)treatments;the fresh and dry weights under red and blue composite light treatments of RB2,RB4 and RB8(rati-os of red to blue light were 2∶1,4∶1 and 8∶1,respectively)were significantly higher than those under R,B and control treatments.(3)Soluble sugar content of gerbera plantlets under RB4 was significantly higher than that of other treatments,increasedby 89.62%comparedto thecontrol.(4)The malondialdehyde content of gerbera plantlets under LED composite light treatments was significantly lower than that of control,and decrease with the increase of red light ratio.The superoxide dismutase activity under RB2,RB4 and RB8 was significantly lower than those under other treatments.Catalase activity of gerbera plantlets under all treatments of LED light qualities,except R,was significantly higher than that of control,and it was the highest under RB4 treatment.The present study indicated that RB2,RB4 and RB8 treatments help to improve multiplication factor and dry and fresh weight,increase the content of soluble sugar,enhance the scavenging active oxygen,alleviate the membrane lipid peroxidation of the gerbera plantlets.Gerbera plantlets had the best performance under RB4 treatment,and they had significantly higher multiplication rate and soluble sugar content than that of other treatments,the highest dry and fresh weight and catalase activity,the lowest malondialdehyde content except red light treatment.Therefore,red and blue LED light ratio 4∶1 is the optimal light quality for proliferation culture of gerbera.更多还原