【摘要】 目的:研究儿茶素对顺铂(DDP)所致人胚肾细胞(HEK293)氧化损伤的影响并探讨其作用机制。方法:体外培养HEK293细胞,分为对照组、DDP组、儿茶素组、儿茶素(5、10、20 mg/L)+DDP组。MTT法检测儿茶素和DDP分别作用后的HEK293细胞存活率,以及儿茶素预处理对DDP所致细胞存活率下降的影响。黄嘌呤氧化酶法检测细胞超氧化物歧化酶(SOD)活力,硫代巴比妥酸法测定细胞内丙二醛(MDA)含量,二硫代二硝基苯甲酸法测定还原型谷胱甘肽(GSH)的含量。结果:与对照组比较,随着浓度的增加,DDP导致HEK293细胞存活率逐渐降低,儿茶素导致细胞存活率先增加后降低。儿茶素预处理后,对DDP(20 mg/L)所致细胞存活率的抑制作用未见明显减弱(P>0.05),但与DDP组相比,能显著抑制DDP听致细胞内MDA含量的升高以及SOD活力和GSH含量的下降(P<0.05)。结论:体外培养条件下,儿茶素预处理虽不能改变DDP对HEK293细胞存活率的影响,但可在一定程度上缓解DDP所致HEK293细胞的氧化损伤。更多还原

【Abstract】 OBJECTIVE:To investigate the mechanism and effect of catechin on cisplatin(DDP)-induced oxidative damage in HEK293 cells.METHODS:Survival HEK293 cells in vitro after their exposure to catechin or DDP was determined.In addition,the effect of catechin pretreatment on DDP-induced cell growth inhibition was determined by the MTT method.Superoxide dismutase(SOD) activity was examined by the xanthine oxidase method.Malondialdehyde(MDA) content was measured by the thiobarbituric acid method.Glutathione(GSH) content was determined by the nitrobenzoic acid method.RESULTS:Cell survival rate decreased gradually as the DDP concentrations increased.Pretreatment with catechin caused increased cell survival initially but decreased gradually afterward.The initial increase was not significant(P>0.05).However,catechin treatment significantly increased the intracellular MDA content,but decreased the SOD activity and GSH level compared with that in the DDP control group(P<0.05).CONCLUSION:Although catechin pretreatment did not improve the survival rate of DDP-treated HEK293 cells,it alleviated the DDPinduced oxidative damage.更多还原