【摘要】 目的 :建立高效液相色谱法测定清平颗粒中人参皂苷Rg₁含量。方法:色谱柱为Agilent ZORBX Eclipse plus C18（4.6 mm×100 mm,3.5μm）,流动相:乙腈（A）-水（B）,检测波长:203 nm,流速:1.0 m L/min,柱温:25℃,进样量:10μL。结果:人参皂苷Rg₁在0.013 2⁰.423 0 mg/m L范围内呈良好线性关系,r=1.000 0;回收率:98.0%,RSD:0.3%。结论:该测定方法操作简便,准确可行,可用于清平颗粒中人参皂苷Rg₁的定量分析。更多还原

【Abstract】 Objective: To establish a HPLC method for determination of ginsenoside Rg₁ content in qingping keli.Methods: Separation was carried out on an Agilent ZORBX Eclipse plus C18（4.6 mm × 100 mm, 3.5 μm）column using acetonitrile（A）-water（B） as mobile at a detection wavelength of 203 nm and a flow rate of 1.0 m L/min. The column temperature was 25 ℃ while the sample load was 10 μL. Results: The linear range of ginsenoside Rg₁ concentration was 0.013 2 0.423 0 mg/m L（r = 1.000 0）. The average recovery of sample was 98.0%, with a RSD of 0.3%.Conclusion: This method is simple, accurate and feasible, which may be used for the quantitative determination of ginsenoside Rg₁ in qingping keli.更多还原