【摘要】 以北高丛蓝莓‘莱格西’（Vaccinium corymbosum cv.Legacy）组培苗为试材,通过正交试验设计研究了不同基础培养基、不同浓度的吲哚丁酸（indole-3-butyric acid,IBA）、活性炭以及浸蘸IBA的浓度对蓝莓组培苗瓶内生根的影响。研究结果表明,蓝莓组培苗瓶内生根的最佳方式为:将蓝莓组培苗在300 mg·L^-1 IBA中浸蘸5 s后,接种到基本培养基——改良1/3WPM+4 mg·L^-1 IBA+300 mg·L^-1活性炭上培养。其中,不同含量的基本培养基组分为促进蓝莓组培苗瓶内生根的主要影响因素,其次为活性炭和浸蘸IBA浓度,而培养基中添加IBA的浓度作用相对较小。更多还原

【Abstract】 In this study,the effect of different kinds of basic medium combined with different concentrations of indole-3-butyric acid（IBA） and active carbon on the rooting rate in Vaccinium corymbosum cv.Legacy（‘Legacy’ blueberry） tissue culture has been tested through the orthogonal test.The results show that the optional combination of test-tube rooting with blueberry shoots was 1/3WPM medium supplemented with 4 mg·L^-1 IBA and 300 mg·L^-1 activated carbon（AC）,and the blueberry shoots in vitro was dipped in 300 mg·L^-1 IBA for 5 seconds in advance.Based on the results,we concluded that the component of basic medium is the main factor for rooting rate promoting on blueberry,following by the concentration of AC and the IBA for dipping,and the effect of IBA addition in basic medium is relatively lower than the former.更多还原