【摘要】 目的:建立以高效液相色谱法同时测定羚羊清肺散中绿原酸、栀子苷、芍药苷和甘草苷的含量的方法。方法:采用双波长HPLC法,Kromasil C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%磷酸水梯度洗脱,流速1.0 m L·min-1,检测波长327 nm(绿原酸),237 nm(栀子苷、芍药苷、甘草苷)。结果:绿原酸、栀子苷、芍药苷和甘草苷4种成分进样量分别在1.579~78.96μg(r=0.999 8),1.261~63.04μg(r=1),0.364~18.2μg(r=0.999 9),0.329 6~16.48μg(r=0.999 9)与峰面积线性关系良好;平均回收率分别为97.6%,97.6%,97.1%和99.8%,RSD分别为1.0%,0.7%,1.2%和1.3%。结论:该方法简便、快速、准确,可用于羚羊清肺散的质量控制。更多还原

【Abstract】 Objective: To establish a HPLC method for simultaneously determining the contents of chlorogenic acid, gardenoside, paeoniflorin and liquiritin in Lingyang Qingfei powder. Method: A double wavelength HPLC method was performed on Kromasil C18column( 4. 6 mm × 250 mm,5 μm) with acetonitrile and0. 1% phosphoric acid solution as the mobile phase for gradient elution. The flow rate was set at 1. 0 m L·min- 1and the column temperature was 28 ℃. The detector wavelength was 327 nm for chlorogenic acid and 237 nm for gardenoside,paeoniflorin and liquiritin. Result: The chlorogenic acid,gardenoside,paeoniflorin and liquiritin showed good linear relationship with peak area at the sample size of 1. 579-78. 96 μg( r = 0. 999 8),1. 261-63. 04 μg( r = 1),0. 364-18. 2 μg( r = 0. 999 9),and 0. 329 6-16. 48 μg( r = 0. 999 9) respectively. The average recovery rate was 97. 6%,97. 6%,97. 1% and 99. 8% respectively for the above components, with RSD of 1. 0%, 0. 7%, 1. 2% and 1. 3%, respectively. Conclusion: The method is simple, rapid and accurate,and can be used for the quality control of Lingyang Qingfei powder.更多还原