【摘要】 目的:探讨马蔺子素对SD大鼠放射性肺损伤的防护作用。方法:将210只SD大鼠随机分为0.9%氯化钠溶液组（正常组）,马蔺子素组（单药组）,单纯照射组（模型组）,激素组,马蔺子素大、中、小剂量(60,30,15 mg·kg^-1·d^-1)组,每组30只。采用⁶⁰Coγ单次20 Gy右全肺照射,建立大鼠放射性肺损伤模型。照射后第1,4,8,12,24周取大鼠肺组织苏木素伊红（HE）染色,马松三色（Masson）染色进行组织病理学观察;紫外分光光度计检测肺组织中羟脯氨酸含量;酶联免疫吸附测定法（ELISA）检测血清肿瘤坏死因子-α（TNF-α）,白细胞介素-1β(IL^-1β)和转化生长因子-β₁（TGF-β₁）浓度;免疫组化法检测肺组织中TNF-α,TGF-β₁的表达。结果:与模型组比较,大、中剂量马蔺子素照射组可显著降低放射性肺损伤的程度,且有降低羟脯氨酸含量和抑制TNF-α,IL^-1β,TGF-β₁表达的作用（P<0.05）。单药组各项指标与正常组相当。结论:中、大剂量马蔺子素对放射性肺损伤有较好的防护作用,且对大鼠肺组织几乎无毒性。更多还原

【Abstract】 Objective: To investigate the protective effect of irisquinone for radiation-inducedlung injury（ RILI） in SD rats. Method: Two hundred and ten SD rats were randomly divided into 0. 9% sodium chloride solution group（ normal group）,irisquinone group（ single drug group）,radiation group（ model group）,hormone group,high-dose( 60 mg·kg^-1·d^-1),medium-dose( 30 mg·kg^-1·d^-1),and low-dose( 15 mg·kg^-1·d^-1)irisquinone + radiation group,n = 30 in each group. The models of RILI in SD rats were established by irradiating right whole chest with a single dose of 20 Gy using⁶⁰ Co γ therapeutic machine. Rats were sacrificed from the seven groups at week 1,4,8,12 and 24 post-irradiation,and the pathological changes of the lung were observed by hematoxylin-eosin（ HE） staining and Masson staining; content of hydroxyproline in lung tissues was detected by UV spectrophotometer,tumor necrosis factor-α（ TNF-α）,interleukin-1β( IL^-1β) and transforming growth factorβ₁（ TGF-β₁） in serum were detected by enzyme-linked immunosorbent assay,expression of TNF-α and TGF-β₁in lung tissues were detected by immunohistochemical method. Result: High-dose and medium-dose irisquinone +radiation group could significantly ameliorate radiation-inducedlung injury, effectively reduce the content of hydroxyproline and inhibite the expression of TNF-α,IL^-1β and TGF-β₁,with statistically significant difference compared with model group（ P < 0. 05）. There was no significant difference between the low-dose irisquinone +radiation group and the model group. There was no statistically significant difference between the single drug group and the normal group. Conclusion: The high-dose and medium-dose irisquinone has protective effect on the radiation-induced lung injury and is nearly non-toxic for the lung tissues of rats.更多还原