【摘要】 采用硅胶、C-18反相硅胶、Sephadex LH-20等柱色谱及高效液相色谱技术对真菌Alternaria sp.XZSBG-1的次级代谢产物进行系统研究;采用波谱分析及与文献比对等方法鉴定化合物结构;采用MTT法和比色法进行抗肿瘤和酶抑制活性筛选。结果从真菌Alternaria sp.XZSBG-1的发酵物中分离得到8个四氢蒽醌类化合物,分别鉴定为Alterporriol F’(1)、Alterporriol G’(2)、Alterporriol F(3)、Alterporriol G(4)、Altersolanol A(5)、Altersolanol F(6)、Tetrahydroaltersolanol B(7)和Tetrahydroaltersolanol D(8);活性测试结果表明仅化合物6对结肠癌细胞株(HCT-116)和子宫癌细胞株(Hela)具有明显抑制活性,IC50值分别为3.026和8.094μmol/L。更多还原

【Abstract】 To study the secondary metabolites of Alternaria sp. XZSBG-1 from Tibet. The chemical compounds were isolated and purified by silica gel,Sephadex LH- 20,ODS- 18 column chromatography,and HPLC. Their structures were characterized by NMR and MS analyses. The antitumor and enzyme inhibitory activities were investigated using MTT and colourimetry methods,respectively. Eight tetrahydroanthraquinone derivatives were isolated from the extract of the fungal strain Alternaria sp. XZSBG-1,and their structures were determined to be Alterporriol F’( 1),Alterporriol G’( 2),Alterporriol F( 3),Alterporriol G( 4),Altersolanol A( 5),Altersolanol F( 6),Tetrahydroaltersolanol B( 7) and Tetrahydroaltersolanol D( 8). Only the compound 6 showed obvious inhibitory activity against HCT-116 and He La cell lines with the IC50 values 3. 026 and 8. 094 μmol / L,respectively.更多还原