【摘要】 通过免疫组化方法检测黑质酪氨酸羟化酶阳性神经元的变化,HPLC-ECD检测纹状体内多巴胺(DA)及其代谢产物含量变化,Western blot检测黑质酪氨酸羟化酶的表达,探讨雷沙吉兰对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱发C57BL/6小鼠帕金森病模型的疗效和行为学影响。结果显示,腹腔注射MPTP的小鼠出现一系列的急性行为学改变,雷沙吉兰(20 mg/kg)治疗后黑质致密部酪氨酸羟化酶阳性细胞数目较模型组显著增加,纹状体内DA及DA代谢产物HVA和DOPAC含量也明显升高,雷沙吉兰明显改善MPTP诱导帕金森小鼠的运动行为学障碍,对帕金森小鼠模型的多巴胺系统具有保护作用。更多还原

【Abstract】 This study aimed at investigating the neuroprotective effect and behavior improvement of rasagiline on1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) model mice of Parkinson’s disease.The tyrosine hydroxylase(TH)-positive dopaminergic neurons in substantia nigra were observed by immunocytochemistry.HPLC-ECD was used to detect the dopamine and its metabolite levels.Western blot was used to examine the protein expression of TH.The results showed that the mice appeared a series of acute behavior change after the injection of MPTP.Rasagiline(20 mg/kg) exerted significant protection against MPTP-induced loss of TH-positive dopaminergic neurons.The TH-positive neurons in rasagiline-treated mice brain increased significantly compared with those of MPTP-treated group.Rasagiline also enhanced dopamine and its metabolite levels in striatum significantly.In conclusion,rasagiline has protective effect on the acute mouse model of MPTP-induced Parkinsonism.更多还原