【摘要】 目的:优选紫苏叶提取液的澄清纯化工艺。方法:比较3种澄清纯化方法即壳聚糖絮凝澄清、ZTC 1+1-Ⅱ絮凝澄清、水沉法对紫苏叶提取液中总黄酮保留率和固形物去除率的影响,筛选出适宜的澄清纯化方法;以总黄酮保留率和固形物去除率的综合评分为指标,分别设计单因素和正交试验考察选定的澄清纯化方法中药液浓缩比、絮凝剂加入量、絮凝温度、搅拌速度等条件的最优值。结果:3种方法中以壳聚糖絮凝澄清法较优;最优澄清纯化工艺为药液浓缩比1∶4、壳聚糖加入量1.0 g/L、絮凝温度60℃、搅拌速度100 r/min、搅拌时间4 min、静置时间12 h;此工艺下总黄酮保留率为(85.1±0.75)%、固形物去除率为(24.6±1.33)%(n=5)。结论:紫苏叶提取液的纯化除杂可选用壳聚糖絮凝澄清法,优选的澄清纯化工艺条件稳定、可行。更多还原

【Abstract】 OBJECTIVE:To optimize the clarification and purification technology of Perillae folium extract.METHODS:The effects of 3 clarification and purification methods as chitosan flocculation clarification,ZTC 1+1-Ⅱflocculation clarification,water precipitation on retention rate of total flavonoids and removal rate of solid of Perillae folium extract were compared to screen suitable clarification and purification technology.With the retention rate of total flavonoids and removal rate of solid as comprehensive evaluation index,single factor and orthogonal test were designed to investigate the optimal value of concentration proportion,the amount of the flocculant,flocculation temperature and whisking speed in optimal clarification and purification method.RESULTS:Among 3 methods,the chitosan flocculation clarification was the best with concentration proportion of 1 ∶ 4,chitosan of 1.0 g/L,flocculation temperature at 60 ℃,whisking speed of 100 r/min,whisking time of 4 min,standing time of 12 h.Under the condition of optimal processing,the retention rate of total flavonoids was(85.1±0.75)%,and the removal rate of solid was(24.6±1.33)%(n=5).CONCLUSIONS:Chitosan flocculation can be used to effectively remove the impurity of Perillae folium extract,and optimized clarification and purification technology is stable and feasible.更多还原