【摘要】 为研究高致病性猪繁殖与呼吸综合征病毒(HP-PRRSV)Hu N4株诱导断奶仔猪胸腺细胞凋亡的分子机制,本实验通过免疫荧光技术、流式细胞术、荧光定量PCR和western blot等方法检测HP-PRRSV感染仔猪胸腺内细胞凋亡相关因子Caspase-8、Caspase-9和Caspase-3表达量的变化。结果显示:HP-PRRSV感染仔猪后,胸腺内表达凋亡相关蛋白Caspase-8、Caspase-9和Caspase-3的细胞比例显著升高,并且凋亡相关蛋白Caspase-8、Caspase-9和Caspase-3表达量也明显升高。以上结果表明:HP-PRRSV感染仔猪引起胸腺细胞凋亡是通过Caspase依赖性通路介导。本研究为进一步了解HP-PRRSV感染机制提供了实验依据。更多还原

【Abstract】 To study the molecular mechanisms of thymocytic apoptosis in the piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus(HP-PRRSV), the changes of apoptosis related proteins in the thymi of HP-PRRSVinfected piglets were investigated by real-time quantitative PCR, flow cytometry, western blot and immunofluorescence methods.The result showed that the caspase-8, caspase-9 and caspase-3 were up-regulated in the piglets infected with HP-PRRSV, and the proportion of cells expressing caspase-related protein increased significantly, Which indicated the extrinsic/intrinsic pathway were activated. These results demonstrated that HP-PRRSV-induced apoptosis through caspase-mediated pathways in thymi of infected piglet.更多还原