【摘要】 通过比较柔嫩艾美耳球虫敏感株与耐药株之间DNA条带的差异来分析耐药性,寻找鸡球虫耐药性虫株的分子标记。采用随机扩增多态性DNA(RAPD)技术对安徽省阜南、舒城、和县、全椒、凤台这5个地区的柔嫩艾美耳球虫和2株敏感株进行遗传多样性分析,同时用5种常用抗球虫药分别对这5个野外分离株进行鸡体试验,5个虫株对上述5种药物均有不同程度的耐药性。RAPD结果显示:5个样品都有相似而清晰的主带,每个泳道有1~25条带不等,大小为2 kb以下。南京敏感株与上海敏感株之间的相似性最高,为78.57%,全椒株与和县株最低且上海敏感株和凤台株最低,均为40.00%,其余虫株之间的相似性处于两者之间,株间平均值为53.85%。5个地理株与2个敏感株之间的SI值也处于较低水平,表明耐药虫株的基因处于较高的变异水平,存在丰富的遗传多样性。更多还原

【Abstract】 This study aimed to analyze drug resistance and to look for the molecular markers for resistant strains in chicken by comparing the difference of DNA bands between the Eimeria tenella sensitive strains and resistant strains. The genetic diversity among two sensitive strains and geographic populations of five different Eimeria tenella collected from Funan, Shucheng, Hexian, Quanjiao and Fengtai in Anhui were analyzed by RAPD. At the same time, chicken body test method was selected to study the resistance to five commonly used anticoccidial drugs. The results showed that the five strains had a different degrees of resistance to these drugs. RAPD results show that five samples had a similar and clear main belt, each lane with 1 to 25 bands ranging in size below 2kb. The similarity coefficient between Nanjing sensitive and Shanghai sensitive strains was highest(78.57%), but Quanjiao and Hexian had lowest similarity value(40%). The remaining strains were 40%—78.57%, with an average of 53.85%.The similarity value between five geographic strains and two sensitive strains remained low, which indicated that the genes of drug resistant strains are at a high level of variation, and there is abundant genetic diversity.更多还原