【摘要】 目的探讨原花青素(procyanidins,PC)对局灶性脑缺血大鼠细胞凋亡和鼠脑组织TNF-α、HSP70和含量的影响。方法选用健康SD大鼠30只,雌雄各半,随机分为假手术组、模型组、PC50 mg/kg组、100 mg/kg组和200 mg/kg组,共5组。24 h后进行神经功能评分,并断头取脑,制备组织匀浆,检测大鼠脑细胞凋亡率、肿瘤坏死因子-α(TNF-α)和热休克蛋白70(HSP70)含量。结果模型组神经功能评分和大鼠脑细胞凋亡率、TNF-α高于假手术组,差异有显著性(P<0.05);模型组大鼠脑组织HSP70含量低于假手术组,差异有显著性(P<0.05)。PC 50 mg/kg组、100 mg/kg组和200 mg/kg组的神经功能评分和大鼠脑细胞凋亡率、TNF-α低于模型组,差异有显著性(P<0.05);HSP70含量高于模型组,差异有显著性(P<0.05)。结论 PC对缺血性脑损伤具有保护作用,其保护作用机制可能与其抑制鼠脑细胞凋亡,并抑制TNF-α的生成,增加HSP70的生成有关。更多还原

【Abstract】 Objective To observe the effect of procyanidins on neural cell apoptosis and the content of TNF-α,HSP70 in rat with focal cerebral ischemia. Methods 30 healthy SD rats,half male and half female,were randomly divided into the sham-operative group,focal cerebral ischemia model group and procyanidins group that was subdivided into 3 groups with 50 mg / kg、100 mg / kg and 200 mg / kg procyanidins respectively. The neurological deficit score of rats was assessed and rat brain cell apoptosis rate,the content of TNF-α and HSP70 in brain tissue were measured 24 h after focal cerebral ischemia. Results The neurological deficit score of sham-operative group was better than that of model group,the percentage of apopotisis cells and the content of TNF-α were lower in sham-operative group than in model group,the content of HSP70 was higher in sham-operative group than that of model group( P < 0. 05). The neurological deficit score were better in these procyanidins preconditioning group with 50 mg / kg,100 mg / kg and 200 mg / kg than that of model group( P < 0. 05). The percentage of apopotisis cells and the content of TNF-α were lower in these procyanidins preconditioning group with50 mg / kg,100 mg / kg and 200 mg / kg than in model group,the content of HSP70 were higher in these procyanidins preconditioning group than that of model group( P < 0. 05). Conclusions Procyanidins that has been given prior to the focal cerebral ischemia plays a neuroprotect role by decreasing the content of TNF-α and apopotisis,increasing the content of HSP70.更多还原