【摘要】 以亚硒酸钠为无机硒源,研究硒的加入对荷叶离褶伞菌丝体胞外粗多糖的影响并优化其发酵条件,选择培养基灭菌条件、添加硒浓度、发酵时间和初始pH值作为优化因素,研究硒在各因素不同水平下对荷叶离褶伞胞外粗多糖含量的影响。通过单因素试验和响应曲面法得到最佳发酵条件:在121℃,20 min对培养基进行灭菌,发酵液中添加2μg/m L Na₂SeO₃,发酵244 h,培养基初始pH值为7.0的条件下,发酵产生的胞外粗多糖含量为0.760 g/100 m L,较未加入硒（0.218 g/100 m L）和加硒未优化前的含量（0.755 g/100 m L）有所提高。研究结果表明,响应曲面法优化富硒荷叶离褶伞发酵条件可使胞外粗多糖含量大幅度提高。更多还原

【Abstract】 Sodium selenite was used as inorganic selenium source,in order to find the optimal fermentation condition of crude exopolysaccharides from selenium-enriched Lyophyllum decastes mycelium. It was carried out to investigate the effects of sterilizing condition,selenium adding amount,incubation time and pH on the yield of crude exopolysaccharides. Influence of different levels of the factors on exopolysaccharides of enriched selenium L. decastes mycelium were studied. The process parameters were optimized using single factor tests and response surface method. The optimal conditions were obtained as follows: sterilizing condition was 121 ℃,20 min,incubation time was 244 h,selenium adding amount was 2 μg/m L Na2 Se O3,and pH was 7. 0. Under these conditions,the extraction yield of exopolysaccharides of enriched selenium L. decastes mycelium was 0. 760 g/100 m L,higher than sample without adding selenium in medium( 0. 218 g/100 m L) and before optimizing fermentation condition( 0. 755 g/100 m L).更多还原