【摘要】 目的 应用GeXP多重基因表达遗传分析系统多重RT-PCR技术检测急性呼吸道感染儿童呼吸道样本中的呼吸道病毒并探讨其在儿童呼吸道感染病毒检测中的价值。方法 回顾性检测2010年7月~2012年6月期间因疑似急性呼吸道感染就诊的1 800名儿科患者的呼吸道样本,应用GeXP多重基因表达遗传分析系统多重RT-PCR技术对呼吸道合胞病毒、鼻病毒等多种呼吸道病毒进行检测。采用SPSS 13.0统计软件进行统计学分析。结果 GeXP多重RT-PCR方法检测到样本中的10种呼吸道病毒。样本中共有67.33%(1212/1800)的样本至少有一种病毒检测结果 为阳性。这1212份病毒阳性标本中,人类鼻病毒(HRV)阳性最多,为375份,阳性率20.83%;其次为呼吸道合胞病毒(RSV)249份,阳性率13.83%;其他依次为人类腺病毒(HADV)、人类副流感病毒3(HPIV-3)、人类博卡病毒(HBoV)、甲型流感(InfA)、人类副流感病毒4(HPIV-4)、流感C(InfC)、人类偏肺病毒(hMPV)和乙型流感病毒(InfB);阳性率分别为8.89%,6.5%,5.3%,5.3%,3.3%,1.5%,1.11%和0.67%。不同性别间患儿呼吸道病毒阳性率差异无统计学意义(P>0.05)。研究中观察到13.5%(243/1 800)的病例同时感染两种或两种以上的病毒,其中两种病毒的混合感染为210例(11.67%),3种病毒混合感染为33例(1.83%)。呼吸道病毒的感染率存在季节差异,RSV大多在春季和冬季流行,HBoV,hMPV,InfA,Inf B和HPIV-3大多是在春季流行,而HADV和HPIV-4主要在夏季流行,Inf C大多是在秋季流行。结论 GeXP分析系统多重RT-PCR方法 是一个快速、高通量、高灵敏度且特异度强的检测分析方法,可用于急性呼吸道感染临床分子诊断和流行病学研究。更多还原

【Abstract】 Objective To detect respiratory viruses in Nasopharyngeal aspirates(NPA) samples from pediatric patients who suspected acute respiratory infection by multiplex reverse transcription-PCR(RT-PCR) with a GenomeLab Gene Expression Profiler(GeXP) analyzer.Methods Selected NPA specimens of 1 800 pediatric patients who suspected acute respiratory infection from July 2010 to June 2012,and tested for including respiratory syncytial virus(RSV),human rhinovirus(HRV)and other respiratory viruses by multiplex reverse transcription-PCR(RT-PCR) with a GenomeLab Gene Expression Profiler(GeXP) analyzer.Datas were analyzed using statistical software(SPSS,version 13.0).Results Totally,ten kinds of respiratory viruses were detected by GeXP genetic analysis system.A total of 67.33%(1 212/1 800) of samples were positive for at least one virus.The 1 212 positive specimens included 375 human rhinovirus(HRV),the positive rate of 20.83%,249 respiratory syncytial virus(RSV),the positive rate of 13.83%,other in turn human adenovirus(HADV).human parainfluenza 3(HPIV-3),human bocavirus(HBoV),Influenza A,human parainfluenza 4(HPIV-4),Influenza C,human metapneumovirus(hMPV) and Inf B.The positive rate was 8.89%,6.5%,5.3%,5.3%,3.3%,1.5%,1.11%and 0.67%respectively.There was no significant difference in the positive rate of respiratory virus among children with different sex(P>0.05).Coinfection by at least 2 of the viral pathogens under study was observed in 13.5%cases(243/1 800).Two pathogens were detected in 210 samples(11.67%) and three pathogens in 33 samples(1.83%).The rates of detection of the viruses were not equally distributed during different seasons.RSV were most detected during the spring and winter,and HBoV,hMPV,InfA,InfB,and HPIV-3 were mostly detected during the Spring,while HADV and HPIV-4 mostly detected during Summer while Inf C were mostly detected in autumn.Condasion The proposed GeXP-based mRT-PCR assay possessed the advantage of rapidity,high throughput,sensitivity and specificity,and could be used for acute respiratory tract infection in clinical molecular diagnosis and epidemiology research.更多还原