【摘要】 α-促黑素细胞激素(α-melanocyte-stimulating hormone,α-MSH)与黑皮质素1受体(melanocortin 1 receptor,MC1R)结合,通过c AMP信号通路影响黑色素的生成。然而,不同浓度α-MSH对绵羊黑色素生成的具体影响,还没有定论。本文旨在探讨不同浓度α-MSH对绵羊皮肤黑素细胞增殖和黑素合成的影响。实验组α-MSH(0、0.1、1、10、100、1 000 nmol/L)和空白对照组相比,荧光定量PCR和Western印迹检测出MC1R、小眼畸形相关转录因子(microphthalmia-associated transcription factor,MITF)和酪氨酸酶(tyrosinase,TYR),在添加10 nmol/Lα-MSH时,基因mRNA水平和蛋白水平的表达量最高,之后则降低;ELISA方法检测出c AMP含量总体增加,c GMP含量总体降低,添加10 nmol/Lα-MSH时,c AMP的产量增加最多,c GMP的产量降低最多;酶标法检测出10 nmol/Lα-MSH时,黑色素生成最多,之后则降低。上述结果证明,不同浓度α-MSH通过调控黑色素形成的c AMP路径,影响MC1R、MITF和TYR的基因和蛋白表达,从而影响黑色素的合成,以添加10 nmol/Lα-MSH时,对黑色素细胞表型和黑色素含量影响最为显著。更多还原

【Abstract】 α-Melanocyte-stimulating hormone( α-MSH) combines with melanocortin 1 receptor( MC1R) to affect the production and distribution of melanin by c AMP pathway. However,the effects of different concentrations of α-MSH on the melanogenesis in sheep melanocytes have not been conclusive.Here we investigated the effects of α-MSH with different concentrations on the proliferation and melanin synthesis in sheep skin melanocytes. Real-time quantitative PCR and Western blot showed that gene mRNA and protein expression level of MC1 R,microphthalmia-associated transcription factor( MITF) and myrosinase( TYR) was the highest in the melanocytes with 10 nmol / L supplementary, and then decreased,compared with that in the control. ELISA method detected that total yield of c AMP was increased,and the total yield of c GMP was decreased,and c AMP production increased and c GMP production declined with significant difference of 10 nmol / L. Enzyme labeled revealed that melanin synthesis was the highest in the melanocytes with 10 nmol / L supplementary,and then decreased. Theresults above suggested that α-MSH affected melanins production in melanocytes by regulating the c AMP pathway of melanogenesis with downstream of MC1 R,MITF and TYR genes expression,and α-MSH of10 nmol / L played the most important roles in the phenotype and melanogenesis of melanocytes.更多还原