【摘要】 研究氯化钙（CaCl₂）结合季也蒙毕赤酵母（Meyerozyma guilliermondii）处理对由炭疽病菌（Colletotrichum gloeosporioides）引起的芒果采后炭疽病的抑制效果,同时分析CaCl₂对病原菌和酵母拮抗菌生长以及果实抗病性的影响。结果显示:40 g/L CaCl₂可有效提升拮抗菌M.guilliermondii对芒果采后炭疽病的生防效果,其中40 g/L CaCl₂与1×108 CFU/m L M.guilliermondii悬浮液的复合处理比单一处理更能有效地抑制芒果果实采后炭疽病的发生。此外,40 g/L CaCl₂对C.gloeosporioides菌丝的生长具有抑制作用,并能够促进拮抗菌M.guilliermondii在芒果果实伤口处的增殖。与此同时,该复合处理显著诱导了芒果果实抗病相关酶活性的升高。结果表明,40 g/L CaCl₂可以直接通过抑制病原菌的生长和促进拮抗酵母的增殖、以及间接诱导果实抗病性来提升拮抗菌M.guilliermondii对芒果果实采后炭疽病防治效果。更多还原

【Abstract】 The purposes of the present study were to investigate the effect of the combined treatment with calcium chloride and the antagonistic yeast Meyerozyma guilliermondii for control of postharvest athracnose caused by Colletotrichum gloeosporioides on mango fruit and to analyze the effect of CaCl₂ on the growth of the pathogen and yeast as well as the disease resistance. The results indicated that the biocontrol activity of M. guilliermondii at 1 × 108 CFU/m L on anthracnose decay was enhanced by combination with 40 g/L CaCl₂ treatment. The combined treatment with M. guilliermondii and 40 g/L CaCl₂ resulted in a remarkably improved control of the disease in comparison with the treatment with M. guilliermondii or 40 g/L CaCl₂ alone. In addition, 40 g/L CaCl₂ inhibited mycelial growth of pathogens and enhanced the population of M. guilliermondiiin the wounds of mango fruits. Meanwhile, the combined treatment induced higher defense-related enzymes activities than either alone. Thus, these results suggested that 40 g/L CaCl₂ could improve the biocontrol activity of M. guilliermondii on the control of postharvest athracnose decay caused by C. gloeosporioides in mango fruit, directly by inhibiting mycelial growth of pathogens and increasing population size of antagonist and indirectly by enhancing disease resistance.更多还原