【摘要】 采用31P核磁共振光谱法快速测定坚果类食品中的6种磷脂。样品经碾碎后,采用三氯甲烷-甲醇混合溶剂提取其中的脂类物质,用氮气流挥干溶剂后,将残渣直接溶于三氯甲烷-甲醇（2+1）氘代溶剂进行核磁分析。6种磷脂的质量浓度的线性范围为0.05².5g·L^-1。方法加标回收率在96.0%以上,测定值的相对标准偏差（n=5）小于5%。实际样品分析结果显示,磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰肌醇为坚果类食品中3种主要磷脂,含量分别为1.72².92mg·g^-1,0.61².20mg·g^-1和0.72⁴.24mg·g^-1。更多还原

【Abstract】 ³¹P-NMR was applied to the determination of 6phospholipids in nuts.After the sample was crushed,lipids were extracted with a mixture of chloroform and methanol.The extract solution was dried by N₂ blowing,then the residue was dissolved in deuterated chloroform-methanol（2+1）solution and analyzed with 31 PNMR directly.The linearity ranges of 6phospholipids were 0.05-2.5g·L^-1.Recovery rates obtained by standard addition method were more than 96.0%,with RSDs（n=5）less than 5%.Results showed that phosphatidylcholine,phosphatidylinositol and phosphatidylethanolamine were the main phospholipids in nuts,with content in the ranges of 1.72-2.92mg·g^-1,0.61-2.20mg·g^-1 and 0.72-4.24mg·g^-1,respectively.更多还原