【摘要】 目的探讨清金化痰汤对慢性阻塞性肺疾病急性加重期(AECOPD)模型大鼠肺组织叉头翼状螺旋转录因子P3(Foxp3)、维甲酸相关孤核受体γt(RORγt)表达的影响。方法 40只SPF Wistar雄性大鼠随机分为正常组、模型组、清金化痰汤组、克拉霉素组。除正常组外,其余组采取气道滴注脂多糖(LPS)联合烟熏的方法建立AECOPD气道黏液高分泌模型,并同时连续30 d分别给予生理盐水、清金化痰汤(8.4 g/kg)、克拉霉素(50 mg/kg)灌胃。实验第31天,提取大鼠肺组织,制作病理切片,并采用免疫组化法检测肺组织中黏蛋白5AC(Muc5AC)、中性粒细胞弹性蛋白酶(NE)的表达,观察Foxp3和RORγt的表达,免疫印迹法检测肺组织中Foxp3和RORγt的蛋白表达。结果与正常组比较,模型组Foxp3表达量无明显差异(P>0.05),RORγt、Muc5AC、NE表达显著升高,Foxp3/RORγt显著下降(P<0.01);清金化痰汤组与模型组比较,Foxp3表达量无明显差异(P>0.05),RORγt、Muc5AC、NE表达显著降低,Foxp3/RORγt显著升高(P<0.01或P<0.05)。清金化痰汤组与克拉霉素组比较,Muc5AC显著降低(P<0.05)。结论清金化痰汤可显著下调模型大鼠肺组织中RORγt蛋白表达,上调Foxp3/RORγt,这可能是清金化痰汤调节AECOPD黏液高分泌的机制之一。更多还原

【Abstract】 Objective To explore the effect of Qingjin Huatan Decoction on forkhead box P3(Foxp3)and retinoid-related orphan nuclear receptor γt(RORγt) in the lung tissue of airway mucus hypersecretion rat model with acute exacerbation of chronic obstructive pulmonary disease(AECOPD). Methods Altogether 40 male Wistar rats,SPF grade,were randomly divided into normal group,model group,Qingjin Huatan Decoction group and clarithromycin group. AECOPD rat model was induced by intratracheal instillation of lipopolysaccharide(LPS) with passive smoking. From the first day of the experiment,rats of model group were intragastrically administered saline,and those of Qingjin Huatan Decoction group and clarithromycin group took corresponding medicines at dose of 8. 4 g/kg and dose of 50 mg/kg for consecutive 30 days. On day 31,all rats were sacrificed. The thin sections of the rats’ resected lungs were made,the expressions of mucin 5AC(Muc5AC),neutrophil elastase(NE),Foxp3 and RORγt in lung tissue were detected by using immunohistochemical method,and the protein expressions of Foxp3 and RORγt were measured by using Western blot. Results Compared with the normal group,Foxp3 protein expression in model group had no significant difference(P > 0. 05),but expression of RORγt,Muc5 AC and NE increased significantly with Foxp3/RORγt decreased significantly(P < 0. 01). Compared with model group,Foxp3 expression in Qingjin Huatan Decoction group had no significant difference(P > 0. 05),but expression of RORγt,Muc5 AC and NE decreased significantly with Foxp3/RORγt increased significantly(P < 0. 01 or P < 0. 05). Compared with clarithromycin group,Muc5 AC decreased significantly in Qingjin Huatan Decoction group(P < 0. 05). Conclusion The potential mechanism of Qingjin Huatan Decoction against AECOPD mucus hypersecretion was to downregulate RORγt protein expression in lung tissue and to upregulate Foxp3/RORγt.更多还原