【摘要】 目的了解江苏省2010-2013年监测标本小肠结肠炎耶尔森菌检出情况,及菌株毒力基因携带情况。方法对2010-2013年监测标本进行菌株分离培养,并对分离菌株5种毒力基因进行检测。结果 2010-2013年共采集各类标本6 335份,检出小肠结肠炎耶尔森菌239株,检出率为3.77%,各年份依次为4.61%,4.65%,3.91%、2.00%,差异有统计学意义(P<0.05)。9种标本中均有检出,以猪便中检出率最高(10.39%),其次为狗便(8.32%)。ail、ystA、ystB、yadA、virF 5种毒力基因检出率分别为0.77%、0.74%、2.48%、0.52%、0.54%,且在猪便、狗便、人便来源菌株中均有检出,除ystB在狗便中检出率最高外,其余均在猪便中检出率最高。结论 2010-2013年江苏省小肠结肠炎耶尔森菌流行趋势较为平稳,宿主来源广泛,其中猪是最重要宿主。更多还原

【Abstract】 Objective To study detection rate of Y.enterocolitic and virulence gene distribution in surveillance samples from 2010 to 2013in Jiangsu Province.Method Samples were cultured to isolate the Y.enterocoliticastrains which were analyzed for 5virulence genes.Result A total of 6 335 samples were collected from 2010 to 2013,among which 239 strains of Y.enterocolitica were indentified,resulting detection rate of 3.77%.The annual detection rate was 4.61%,4.65%,3.91%and 2.00%,respectively,with statistical difference(P<0.05).Y.enterocolitica was isolated from 9kind of specimens;the highest detection rate was observed amomg pig stool samples(10.39%),followed by dog stool samples(8.32%).The detection rate of 5virulence genes of ail、ystA、ystB、yadA、virF5was 0.77%,0.74%,2.48%,0.52% and 0.54%,respectively.All virulence genes were detected from strains isolated from pig/dog/human stool samples.Except for ystB had highest detection rate anong dog stool samples,the highest detection rates for other virulence genes were detected among pig stool samples.Conclusion The prevalence trend of Y.enterocoliticain Jiangsu Province was stable.Moreover,the hosts of Y.enterocolitica were widespread,among which pig was the most important host.更多还原