【摘要】 对来源于黑布林的β-葡萄糖苷酶进行了交联法制备(CLEAs),并对其酶学性质进行了初步研究。结果发现,当异丙醇为蛋白沉降剂时CLEAs的酶活回收率最高达58.9%;该聚合酶的最适温度为55℃,显示出良好的温度耐受性;CLEAs的Km、Vmax和Ki值分别为5.74 m M、203.4μmol/(min·mg蛋白)和394.03 m M;此外,金属离子Hg2+、Ag+可显著降低CLEAs的酶活。更多还原

【Abstract】 The cross- linked enzyme aggregates( CLEAs) were successful prepared based on the β- glucosidase form black plum seed and its enzymology characteristics were studied. The results revealed that the highest enzyme activity recovery of 58. 9% was obtained when isopropyl alcohol was used as the sinking agent. The CLEAs displayed good thermal tolerance with the optimum temperature of 55℃. The Km,Vmax and Ki values of the CLEAs were 5. 74 m M,203. 4 μmol /( min·mg protein) and 394. 03 m M,respectively. Moreover,the influence of metal cations and reagents showed that Hg2 +and Ag+remarkably reduced the enzyme activity.更多还原