【摘要】 目的通过建立新西兰兔的高脂血症模型,观察葡萄籽原花青素(GSPE)对兔动脉粥样硬化(AS)血管病理形态的影响。方法选用新西兰大白兔,随机分为空白对照组、高脂模型组、GSPE小剂量组、中剂量组、大剂量组、洛伐他汀阳性对照组,复制高脂食饵性兔AS模型,每组7只。空白对照组喂饲基础饲料;其余各组给予高脂饲料(80%普通饲料+14%蛋黄粉+1%胆固醇+5%猪油)喂饲,实验共8周。给予高脂饲料第1天起开始给药,GSPE组每兔每天每公斤体重给予0.1 g、0.2 g、0.4 g,洛伐他汀阳性对照组每公斤体重给予洛伐他汀1 mg,以上药物均胶囊给药,模型组、空白对照组给以空胶囊。观察原花青素预防性用药后对兔主动脉内皮损伤的病理形态影响。结果GSPE大、中剂量组与高脂模型组比较,从大体形态、组织学、扫描电镜三方面显示内皮细胞损伤均有一定的改善,斑块/内膜面积比分别减少29.5%,27.2%,差异有统计学意义(P<0.05),内/中膜厚度比分别减少72.5%,60.2%,差异有统计学意义(P<0.05)。对主动脉内膜超微结构也有良好的修复和保护作用。但小剂量GSPE与高脂模型组比较,差异无统计学意义(P>0.05)。结论大、中剂量GSPE对于减轻AS兔主动脉内膜损伤病变有一定作用。更多还原

【Abstract】 Objective To observe the effects of grape seed procyanidin extract( GSPE) on pathological morphous of atherosclerosis(AS) blood vessel in rabbits by means of established animal models with hyperlipidemia.Methods Forty-two New Zealand white rabbits were randomly divided into six groups: blank control group,hyperlipidemia model group,low-dose GSPE group(0.1g/kg),median-dose GSPE group(0.2g/kg),high-dose GSPE group(0.4g/kg) and lovastatin positive control group(1mg/kg),with 7 rabbits in each group.The rabbits in blank control group were fed with normal diet,however,the rabbits in the other 5 groups were fed with high fat diet for 8 weeks to establish AS models,finally,the effects of GSPE on the pathological morphous of aorta endothelium injury of rabbits were observed and evaluated.Results As compared with that in hyperlipidemia model group,the aorta endothelium injury showed by general morphous,histology,scanning electron microscopy in median-dose GSPE group and high-dose GSPE group was improved at some extent,especially,the ratio of atherosclerotic plaque/intima area was significantly decreased by 29.5%,27.2%,respectively(P<0.05).Moreover the ratio of intima/tunica media was obviously decreased by 72.5%,60.2%,respectively(P<0.05).The good recovery and protective effects of GSPE on the ultrastructure of aortic tunica intima were observed in median-dose GSPE group and high-dose GSPE group,but there were no significant differences between low-dose GSPE group and hyperlipidemia model group(P >0.05).Conclusion The high-dose and median-dose GSPE play a certain role in alleviating aortic tunica intima injury of rabbits with AS.更多还原