【摘要】 利用秋水仙素,采用浸泡法和固体培养法分别对黄花蒿(Artemisia annua L.)丛生芽进行多倍体诱导,以期获得黄花蒿多倍体种质。结果表明,以5 000 mg/L秋水仙素浸泡丛生芽5 d处理获得的诱导效果最好,诱导率可达40%。对性状变异明显的黄花蒿植株进行染色体数目观察,发现了黄花蒿四倍体和嵌合体。更多还原

【Abstract】 In order to obtain new polyploid germplasm of Artemisia annua L., immersing and solid culture method with colchicines were used for polyploidy induction. The results showed that the buds immersed in 5 000 mg / L colchicine for 5 d had the highest polyploidy induction rate as 40%. Tetraploid and chimera plants were found by observing the chromosome numbers of A. annua polyploid plants with obvious phenotypic variances更多还原