【摘要】 采用微量细胞板培养法分离流感病毒,RT-PCR、巢式PCR鉴定INFB及其HA1基因,运用Clustax、Mega 6.0、Megalign软件分析HA1核苷酸序列及氨基酸序列。结果:2014年4月—2015年3月共收集到发热呼吸道样本1 002株,分离到47株INFB阳性,分离出INFB阳性高峰出现在2014年8月和2015年1月,13~24个月儿童INFB阳性率最高,0~6个月儿童阳性率最低。INFB患儿与患儿的性别及科室来源无显著关系,87.23%INFB患儿出现了发热、咳嗽症状。HA1片段分析结果表明,检测到的INFB属于Victoria系,与参考株B/Brisbane/60/2008核苷酸同源性在86.1%~88.0%,与B/HubeiJingzhouSongzi/1302/2010核苷酸同源性在86.1%~89.5%。3个氨基酸位点发生转换。结论:武汉地区儿童感染的INFB属于Victoria系,与北半球国际疫苗株B/Brisbane/60/2008、B/Texas/AF2741/2010以及湖北省INFB代表毒株B/Hubei JingzhouSongzi/1302/2010具有较高的亲缘关系,未发现新变种。更多还原

【Abstract】 Using micro plate method to isolate the influenza virus,Influenza B virus and its HA1 gene were identified by RT-PCR and Nested-PCR,The nucleotide sequence of HA1 gene were analyzed by Clustax,Mega6.0,Megalign.Results:47influenza B positive samples were isolated from 1 002 fever samples collected from April,2014-to March,2015.The highest isolation rate was found in August2014,and January,2015.The highest influenza B positive was found in 13~24months old children,and the lowest positive rate was found in 0~6months old children.There is no significant difference among children patients with influenza B virus infection in either-gender or departments source:87.23% of children of the Influenza B virus infection show the symptoms of fever and cough.HA1 fragment analysis indicates the isolated influenza B virus belongs to the Victoria strain,and shows a 86.1% ~88.0% homology to the reference strain,B/Brisbane/60/2008 in nucleotide sequence.Three amino acids of the isolated strain were different from the reference strain.Conclusion:Influenza B virus isolated in Wuhan belongs to the Victoria strain,which has high homology with the international vaccine strain B/Brisbane/60/2008 and B/Texas/AF2741/2010 in the northern hemisphere and INFB representative strains in Hubei province.No new strain was found.更多还原