【摘要】 本研究以蜻蜓凤梨为材料,利用RACE技术获得一个与拟南芥AGAMOUS(AG)同源的编码基因c DNA,并将其命名为Af AG。Af AG基因的c DNA全长1 422 bp,开放阅读框为723 bp,可翻译成240个氨基酸。利用NCBI对Af AG蛋白进行序列分析,结果显示Af AG蛋白含有MADS-box蛋白家族共有的功能结构域:MADS和K-BOX两个结构域。与水稻(Os AG);玉米(Zm AGL);拟南芥(At AG);菜心(Br AGL);椰子(Cn AGL);白兰花(Ma AGL)的同源性比对结果分别为65%、63%、68%、65%、83%、75%。本研究成功构建了Ca MV35S-Af AG过表达载体,用农杆菌介导的方法将表达载体成功转入到拟南芥中,为进一步研究蜻蜓凤梨开花分子机理提供了理论依据。更多还原

【Abstract】 In this study, a c DNA homolog of AGAMOUS(AG) of Arabidopsis thaliana was isolated using rapid amplification of c DNA ends(RACE) technology from Aechmea fasciata, and named it as Af AG. The full length c DNA fragment of Af AG was 1 422 bp, which has an Open Reading Frame(ORF) of 723 bp and encodes a protein of 431 amino acids. BLASTP analysis showed that Af AG has both MADS box and K box domains, which are conserved in MADS box protein family. Phylogenetic analysis of Af AG indicated that the deduced protein had65% 、63% 、68% 、65% 、83% 、75% sequence identities with Oryza sativa, Zea mays, Arabidopsis, Brassica rapa,Cocos nucifera and Michelia alba, respectively. Based on these, we successfully constructed 35S::Af AG overexpression vector, and screened the positive transgenetic arabidopsis plants. Our studies provide some theoretical research inspects of the flowering mechanism of Aechmea fasciata.更多还原