【摘要】 抗菌肽作为新型的潜在抗菌药物,在耐药问题日益严峻的今天引起了广泛关注。在对抗菌肽体外研究不断深入的同时,抗菌肽体内研究也逐渐成为关注重点。论文主要从抗菌肽的体内抗菌活性研究和毒性评价两个方面进行了综述,在体内抗菌活性研究中介绍了模型设计及动物试验原则、接种途径和模型鉴定方法,并对常用于抗菌肽体内研究的几种典型细菌感染动物模型建立及活性试验方法进行了总结,在毒性评价中主要介绍了测定半数致死量(LD50)法,同时对建模及活性毒性评价研究中的不同方法进行了对比分析。更多还原

【Abstract】 As novel potential antimicrobial agents,antimicrobial peptides cause widespread concern at the growing problem of drug resistance.While in vitro study of antimicrobial peptides is deepening,studies in vivo has become the focus.We reviewed two aspects including antibacterial activity and toxicity in vivo.In vivo antibacterial activity assessment,principles of model design and experimental animals,vaccination approach and model identification method were mainly introduced.The establishment of typical animal models of bacterial infections and the evaluation methods of antibacterial activity were summarized.In the toxicity assessment,the method of median lethal dose(LD50)was mainly introduced.And different methods about two aspects were compared and analyzed.更多还原