【摘要】 以牛骨为原料,采用酶解法提取胶原蛋白,对影响胃蛋白酶酶解过程的诸多因素进行研究;在单因素实验的基础上进行正交优化,得到酶解法提取胶原蛋白的最佳工艺参数。结果表明:最佳酶解条件为脱脂时间6 h,脱钙时间6 h,加酶量0.50%,p H 1.8,酶解温度38℃,酶解时间8 h。在该条件下,牛骨胶原蛋白提取率最高,可以达到25.38%。更多还原

【Abstract】 The collagen of bovine bone has been extracted by using pepsin,and the effect of the main process parameters for the hydrolysis of bovine bone was investigated. On the basis of single factor test,orthogonal design was used to optimize the enzymolysis technology. The results showed that the optimal hydrolysis conditions for extracting collagen from bovine bone included degreasing time is 6 h,decalcifying time is 6 h,enzyme amount is 0. 50%,p H 1. 8,hydrolysis is at 38 ℃,enzymatic hydrolysis time is 8 h,at which the yield of collagen can reach the highest 25. 38% 。更多还原