【摘要】 目的:观察佛甲草提取物对S180小鼠肿瘤组织白介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)及核转录因子-κB(NF-κB/P65、NF-κB/P50)蛋白表达的调节作用。方法:建立小鼠肉瘤S180移植性肿瘤模型,接种次日,将小鼠随机分为模型组、50 mg/kg环磷酰胺组、4 g/kg及8 g/kg佛甲草组,每组10只,连续给药14天。观察小鼠体重变化,计算肿瘤指数;应用蛋白免疫印迹技术(Western blot)检测肿瘤组织IL-10、TNF-α及NF-κB/P65、NF-κB/P50的蛋白表达。结果:经佛甲草提取物4,8 g/kg治疗后,S180荷瘤小鼠肿瘤指数明显低于模型组,抑瘤率分别高达84.26%,62.24%;且能显著提高小鼠S180肿瘤组织IL-10、TNF-α及NF-κB/P65、NF-κB/P50蛋白的相对表达量。结论:佛甲草可能通过激活NF-κB信号通路,上调TNF-α的表达发挥抑瘤作用,另外,佛甲草还可通过提高IL-10水平起抗肿瘤作用。更多还原

【Abstract】 Objective: To study the anti-tumor mechanisms of Sedum lineare Thunb(SLT) alcohol extract by investigate the protein expression of IL-10,TNF-α and NF-κB in tumor tissue in mice bearing S180 sarcoma.Methods: S180-bearing mice model were established by subcutaneously inoculating S180 ascitic tumor into the right armpit.From the second day,the mice were treated with normal saline,cyclophosphamide(50 mg/kg) or two doses of SLE(4,8 g/kg) through intragastric administration,and the process extent 14 days.After the last administration,the mice body weight and the index of tumor,the protein expression of IL-10,TNF-α,NF-κB/P65 and NF-κB/P50 in tumor tissue were measured by western blot method.Results: The body weight of mice treatmented with SLT was dramaticlly higher than those of the CP.Compare with the model group,SLT groups showed decreases in tumors index and could significantly improve the protein expression of IL-10,TNF-α,NF-κB/P65 and NF-κB/P50.Conclusion: Activating NF-κB and then elevating TNF-α level is one of the mechanisms of SLT’s anti-tumor effect,and enhancing IL-10 content is the other one.更多还原