【摘要】 目的观察乌骨藤粗提取物（MTE）不同组分对大鼠C6胶质瘤细胞增殖情况的影响,探讨其作用机制。方法采用新型四氮唑盐法（MTS）检测MTE的Fr1、Fr2、Fr3组分对C6细胞增殖的影响;采用流式细胞仪检测Fr2对C6细胞凋亡和周期的影响;采用荧光定量PCR（qPCR）技术检测不同浓度Fr2对C6细胞的Bax、Bcl-2表达的影响。结果 MTE的Fr1、Fr2、Fr3均能显著抑制C6细胞的增殖（P<0.05）,与阳性对照组5-氟尿嘧啶（5-FU）相比抑制率也较高（P<0.05）,其半数抑制浓度分别为:60.32、52.30、83.83μg/ml。经80、160μg/ml的Fr2处理24 h后,可有效促进C6细胞早期和晚期凋亡（P<0 05）,其周期被阻滞在G₂期;Fr2相同方式处理后,C6细胞中Bax表达水平分别提高到（124.80±7.68）%和（380.30±6.91）%;Bcl-2表达水平分别降低到（84.20±3.10）%和（34.20±4.80）%（P<0.05）。结论 MTE能够显著抑制C6胶质瘤细胞的增殖,其中主要有效成分Fr2能够诱导C6细胞发生G₂期周期阻滞,并通过影响Bax和Bcl-2的表达,促进其凋亡。更多还原

【Abstract】 Objective To investigate the effect of Marsdenia tenacissima extract（MTE） on proliferation of rat glioma C6 cells.Methods A new tetrazolium salt reduction method（MTS） was used to measure the effect of Fr1,Fr2,Fr3 component from MTE on C6 cell proliferation,flow cytometry was applied to measure cell apoptosis and cell cycle,real-time quantitative PCR（qPCR） was used to detect Bax and Bcl-2 gene expression in C6 cells treated by Fr2 with different concentrations.Results Fr1,Fr2 and Fr3 of MTE significantly inhibited the proliferation of C6 cells（P<0.05）,the inhibition rate was higher than that of 5-fluorouracil（5-FU）（P<0.05）,and the 50%inhibitory concentrations were 60.32μg/ml,52.30μ g/ml,and 83.83 μg/ml,respectively.After treated with 80 μg/ml and 160 μg/ml of Fr2 for 24 h,early and late apoptosis of C6 cells were both promoted（P<0.05）,and the cell cycle was arrested at G₂ phase;Bax gene expression was increased to（124.80 ± 7.68）%and（380.30 ± 6.91）%;Bcl-2 gene expression was reduced to（84.20±3.1）%and（34.20 ±4.80）%,respectively（P<0.05）.Conclusion This study suggests that MTE can significantly inhibit the proliferation of C6 glioma cells,and the main active ingredient Fr2 can induce G₂ phase cycle arrest and promote apoptosis,which were associated with changes of Bax and Bcl-2 gene expression.更多还原