【摘要】 目的比较miRNA-140和MMP-3在骨性关节炎(OA)组与非OA组关节滑液中的表达,探究miRNA-140和MMP-3与OA的相关性。方法采用实时定量PCR技术检测miRNA-140、MMP-3在OA组(n=65)与非OA组(n=65)关节滑液中的表达水平,并运用Spearman相关分析分析miRNA-140与MMP-3的相关性。结果组间比较显示OA组miRNA-140的表达显著低于非OA组(P<0.05),MMP-3的表达明显高于非OA组(P<0.05)。OA组内各因子之间相关分析显示,miRNA-140和MMP-3呈显著负相关性(r=-0.693、-0.720,P<0.05)。结论相对于非OA病变,OA组关节滑液中miRNA-140低表达,MMP-3高表达,两者之间呈负相关性。更多还原

【Abstract】 Objective To exploring the correlation between miRNA-140 and MMP-3 in osteoarthritis( OA),this study compared the OA group with non-OA group over the expression of MMP-3 and miRNA-140 in synovial fluid.Methods Using Real-time quantitative PCR to detect the expression levels of miRNA-140,MMP-3 in synovial fluid both OA group( n = 65) and non-OA group( n = 65),then exploring the relationship between miRNA-140 and MMP-3 by using Spearman correlation analysis software. Results The expression of miRNA-140 in OA group was significantly lower than non-OA group( P < 0. 05),while the expression of MMP-3 in OA group was significantly higher than non-OA group( P < 0. 05). miRNA-140 and MMP-3 had a significant negative correlation in OA group by using Spearman correlation analysis software,the correlation coefficient( r =-0. 693、-0. 720,P < 0. 05).Conclusions Relative to non-OA lesions,miRNA-140 shows low expression in OA synovial fluid group,while MMP-3 in the OA group is highly expressed,both showed a significant negative correlation in OA group and nonOA group.更多还原