【摘要】 实验分析了陕西毛乌素沙地典型湖滨湿地土壤理化性质和石油降解菌的分离与鉴定,为沙化环境下湖滨湿地微生物修复石油污染提供科学依据。2006—2013年,在巴吓采当距湖边1m,10m和50m处共采集9个土样,测量了各土样的有机质、硝态氮、铵态氮和全磷等理化性质,分离出5种石油降解菌,利用16S rDNA进行鉴定。湖滨湿地pH值最高值为7.52,最低值为4.88。土壤中有机质、全磷含量分别在3.47~72.36,0.28~1.04g/kg,硝态氮、铵态氮和速效磷含量分别在2.57~14.19,16.53~37.45,3.93~10.26mg/kg。16S rDNA测序比对结果显示,巴吓采当湖滨湿地石油降解菌为蜡样芽胞杆菌(Bacillus cereus)和苏云金芽孢杆菌(Bacillus thuringiensis)。巴吓采当湖滨湿地土壤为偏酸性,土壤肥力比红碱淖地区高。更多还原

【Abstract】 This study aimed to investigate the soilis physical and chemical properties,separate identification of oil degradation bacteria in Mu Us Sandland area,Shaanxi. In 2006—2013,soil samples were collected from1 m,10m,50 m of Baxiacaidang Lakeside. The soil physic-chemical properties,such as soil organic matter,nitrate nitrogen,ammonium nitrogen,available phosphorus,total phosphorus and so on,were analyzed. Five medium were used to isolate the oil degradation bacteria in soil samples and were identified using 16 S r DNA sequence. The soil p H was 7. 52 at highest and 4. 88 at lowest. The contents of soil organic matter and total phosphorus were 3. 47 ~ 72. 36 and 0. 28 ~ 1. 04 g / kg respectively. The contents of nitrate nitrogen,ammonium nitrogen,and available phosphorus were 2. 57 ~ 14. 19,16. 53 ~ 37. 45 and 3. 93 ~ 10. 26 mg / kg respec-tively. 16 S r DNA sequence analysis showed oil degradation bacteria in Baxiacaidang Lake were Bacillus cereus and Bacillus thuringiensis. The result revealed that soil in Baxiacaidang Lake was slightly acidic and higher fertility than Hongjiannao.更多还原